Depigmenting effect of Cinnamomum cassia Presl in B16F10 melanoma cells

To find efficient depigmenting agents, we examined several Chinese herbs for melanogenesis inhibition and toxicity. Cinnamomum cassia Presl exhibited low cytotoxicity at even high concentration (200 mu g/ml). The effects on melanogenesis of cultured B16 melanoma cells, mushroom tyrosinase activity, and free radical scavenging activity were further assessed. The methanol extracts of this plant showed the suppression of melanin synthesis. Melanin content was dose-dependently decreased by this herb extract as compared with control cells. It also showed good anti-oxidative activity (IC50 = 3.7 mu g/ml) but no inhibition of mushroom tyrosinase activity. This result showed that Cinnamomum cassia Presl extract might be useful and safe as a new whitening agent in cosmetics.