Keth-seq for transcriptome-wide RNA structure mapping

被引：83

作者：

Weng, Xiaocheng ^{[1
,2
]}

Gong, Jing ^{[3
]}

Chen, Yi ^{[2
]}

Wu, Tong ^{[1
]}

Wang, Fang ^{[1
,4
]}

Yang, Shixi ^{[2
]}

Yuan, Yushu ^{[2
]}

Luo, Guanzheng ^{[1
]}

Chen, Kai ^{[1
]}

Hu, Lulu ^{[1
]}

Ma, Honghui ^{[1
]}

Wang, Pingluan ^{[1
]}

Zhang, Qiangfeng Cliff ^{[3
]}

Zhou, Xiang ^{[2
]}

He, Chuan ^{[1
]}

机构：

[1] Univ Chicago, Howard Hughes Med Inst, Inst Biophys Dynam, Dept Chem,Dept Biochem & Mol Biol, 5841 S Maryland Ave, Chicago, IL 60637 USA

[2] Wuhan Univ, Minist Educ, Key Lab Biomed Polymers, Coll Chem & Mol Sci, Wuhan, Peoples R China

[3] Tsinghua Univ, Ctr Synthet & Syst Biol, Tsinghua Peking Joint Ctr Life Sci,Sch Life Sci, MOE Key Lab Bioinformat,Beijing Adv Innovat Ctr S, Beijing, Peoples R China

[4] Wuhan Univ, Sch Pharmaceut Sci, Wuhan, Peoples R China

来源：

NATURE CHEMICAL BIOLOGY | 2020年 / 16卷 / 05期

基金：

中国国家自然科学基金; 美国国家卫生研究院;

关键词：

SELECTIVE 2'-HYDROXYL ACYLATION; SECONDARY STRUCTURE; REVEALS;

D O I：

10.1038/s41589-019-0459-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA secondary structure is critical to RNA regulation and function. We report a new N-3-kethoxal reagent that allows fast and reversible labeling of single-stranded guanine bases in live cells. This N-3-kethoxal-based chemistry allows efficient RNA labeling under mild conditions and transcriptome-wide RNA secondary structure mapping.

引用

页码：489 / +

页数：9

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