Chondroitin sulfate cross-linked three-dimensional tailored electrospun scaffolds for cartilage regeneration

被引：31
作者：
Chen, Yujie [1 ]
Xu, Wei [2 ,3 ,4 ]
Shafiq, Muhammad [1 ,5 ]
Song, Daiying [2 ,3 ,4 ]
Xie, Xianrui [1 ]
Yuan, Zhengchao [1 ]
EL-Newehy, Mohamed [6 ]
EL-Hamshary, Hany [6 ]
Morsi, Yosry [7 ]
Liu, Yu [2 ,3 ,4 ]
Mo, Xiumei [1 ]
机构：
[1] Donghua Univ, Coll Chemisny Chem Engn & Biotechnol, Shanghai Engn Res Ctr Nanobiomat & Regenerat Med, State Key Lab Modificat Chem Fibers & Polymer Mat, Shanghai 201600, Peoples R China
[2] Wei Fang Med Coll, Res Inst Plast Surg, Weifang 261000, Peoples R China
[3] Natl Tissue Engn Ctr China, Shanghai 201100, Peoples R China
[4] Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Shanghai Stem Cell Inst, Dept Plast & Reconstruct Surg,Sch Med,Shanghai Ke, Shanghai 200001, Peoples R China
[5] Univ Cent Punjab UCP, Fac Life Sci FLS, Dept Biotechnol, Lahore 54000, Pakistan
[6] King Saud Univ, Coll Sci, Dept Chem, POB 2455, Riyadh 11451, Saudi Arabia
[7] Swinburne Univ Technol, Fac Engn & Ind Sci, Boroondara, Vic 3122, Australia
来源：
BIOMATERIALS ADVANCES | 2022年 / 134卷
基金：
中国国家自然科学基金;
关键词：
Electrospinning; Three-dimensional; Gas foaming; Chondroitin sulfate; Cartilage tissue engineering; Silk fibroin; IN-VITRO; NANOFIBROUS SCAFFOLDS; CHONDROGENIC DIFFERENTIATION; MECHANICAL-PROPERTIES; SUBSTANCE-P; TISSUE; FABRICATION; MATRIX; BONE; CHONDROCYTES;
D O I：
10.1016/j.msec.2022.112643
中图分类号：
TB3 [工程材料学]; R318.08 [生物材料学];
学科分类号：
0805 ; 080501 ; 080502 ;
摘要：
Degenerated cartilage tissues remain a burgeoning issue to be tackled, while bioactive engineering products available for optimal cartilage regeneration are scarce. In the present study, two-dimensional (2DS) poly(L-lactide-co-epsilon-caprolactone)/silk fibroin (PLCL/SF)-based scaffolds were fabricated by conjugate electrospinning method, which were then cross-linked with chondroitin sulfate (CS) to further enhance their mechanical and biological performance. Afterwards, three-dimensional (3D) PI.CL/SF scaffolds (3DS) and CS-crosslinked 3D scaffolds (3DCSS) with tailored size were successfully fabricated by an in-sini gas foaming in a confined mold followed by freeze-dried. Gas-foamed scaffolds displayed high porosity, rapid water uptake, and stable mechanical properties. While all of the scaffolds exhibited good cytocompatibility in vitro; 3DCSS showed better cell seeding efficiency and chondro-protective effect compared to other scaffolds. Besides, 3DCSS scaffolds supported the formation of more mature cartilage-like tissues along with the best repair outcome in a rabbit articular cartilage defect model in vivo, as well as less expression level of pro-inflammatory cytokines, including interleukin (IL)-1 beta and tumor necrosis factor (TNF)-alpha than that of the other groups. Taken together, 3DCSS may provide an alternative therapeutic option for cartilage tissue repair.
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页数：16
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