Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae

被引：8

作者：

Muto, Akira ^{[1
]}

Kawakami, Koichi ^{[1
]}

机构：

[1] SOKENDAI Grad Univ Adv Studies, Natl Inst Genet, Dept Genet, Div Mol & Dev Biol, Hayama, Japan

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2018年 / 136期

关键词：

Neuroscience; Issue; 136; Laser-ablation; zebrafish larvae; calcium imaging; prey capture; feeding; pretectum; hypothalamus; two-photon microscopy; vision; neuron; GCaMP; GFP; SPINAL PROJECTION NEURONS; VISUALIZATION; PERCEPTION; ACTIVATION; CIRCUITS; TECTUM; GENE;

D O I：

10.3791/57485

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To identify the role of a subpopulation of neurons in behavior, it is essential to test the consequences of blocking its activity in living animals. Laser ablation of neurons is an effective method for this purpose when neurons are selectively labeled with fluorescent probes. In the present study, protocols for laser ablating a subpopulation of neurons using a two-photon microscope and testing of its functional and behavioral consequences are described. In this study, prey capture behavior in zebrafish larvae is used as a study model. The pretecto-hypothalamic circuit is known to underlie this visually-driven prey catching behavior. Zebrafish pretectum were laser-ablated, and neuronal activity in the inferior lobe of the hypothalamus (ILH; the target of the pretectal projection) was examined. Prey capture behavior after pretectal ablation was also tested.

引用

页数：11

共 27 条

[21] Spontaneous Neuronal Network Dynamics Reveal Circuit's Functional Adaptations for Behavior [J].

Romano, Sebastian A. ;

Pietri, Thomas ;

Perez-Schuster, Veronica ;

Jouary, Adrien ;

Haudrechy, Mathieu ;

Sumbre, German .

NEURON, 2015, 85 (05) :1070-1085

[22] A dedicated visual pathway for prey detection in larval zebrafish [J].

Semmelhack, Julia L. ;

Donovan, Joseph C. ;

Thiele, Tod R. ;

Kuehn, Enrico ;

Laurell, Eva ;

Baier, Herwig .

ELIFE, 2014, 3

[23] Vesicular glutamate transport at a central synapse limits the acuity of visual perception in zebrafish [J].

Smear, Matthew C. ;

Tao, Huizhong W. ;

Staub, Wendy ;

Orger, Michael B. ;

Gosse, Nathan J. ;

Liu, Yan ;

Takahashi, Koji ;

Poo, Mu-Ming ;

Baier, Herwig .

NEURON, 2007, 53 (01) :65-77

[24]

Sternberg J. R., 2016, CURR BIOL

[25] A pyramid approach to subpixel registration based on intensity [J].

Thevenaz, P ;

Ruttimann, UE ;

Unser, M .

IEEE TRANSACTIONS ON IMAGE PROCESSING, 1998, 7 (01) :27-41

[26] Visually driven chaining of elementary swim patterns into a goal-directed motor sequence: a virtual reality study of zebrafish prey capture [J].

Trivedi, Chintan A. ;

Bollmann, Johann H. .

FRONTIERS IN NEURAL CIRCUITS, 2013, 7

[27]

Westerfield M., 2007, ZEBRAFISH BOOK, DOI DOI 10.14

← 1 2 3 →