Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp piscicida

被引:91
作者
Chabrillón, M [1 ]
Rico, RM [1 ]
Balebona, MC [1 ]
Moriñigo, MA [1 ]
机构
[1] Univ Malaga, Fac Ciencias, Dept Microbiol, E-29071 Malaga, Spain
关键词
bacterial pathogens; Photobacterium damselae subsp; piscicida; probiotics; prophylaxis; Solea senegalensis; Sparus aurata;
D O I
10.1111/j.1365-2761.2005.00623.x
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
Most studies carried out to select microorganisms as candidate probiotics have focused on in vitro antagonism tests, such as the production of inhibitory compounds against pathogenic microorganisms. However, attachment to mucous surfaces could be another criterion to be considered when selecting potential probiotics for aquaculture. Nineteen isolates obtained from farmed Senegalese sole, Solea senegalensis Kaup, and gilthead sea bream, Sparus aurata L., have been evaluated for their capacity to adhere to skin and intestinal mucus of Senegalese sole, and their antagonistic effect against Photobacterium damselae subsp. piscicida, an important pathogen for farmed sole. The isolates from gilthead sea bream showed the highest percentage of adhesion to sole mucus, whilst the pathogenic microorganisms assayed and the isolates from sole showed, in general, a lower ability to adhere to sole mucus. The results suggest that the adhesion to fish mucus was more dependent on the isolate tested than on the host mucus. The isolates from gilthead sea bream also showed a higher antagonistic activity against P. damselae subsp. piscicida than those from Senegalese sole. Four isolates were selected, on the basis of their adhesive ability and antagonistic effect on P. damselae subsp. piscicida, to study their interactions with the pathogen in respect of adhesion to skin and intestinal mucus under exclusion, competition and displacement conditions. The results obtained show the ability of three isolates to reduce the adhesion of P. damselae subsp. piscicida to sole mucus under displacement and competition conditions. The adhesion of the pathogen to sole intestinal mucus was also significantly reduced when three isolates were assayed under exclusion conditions.
引用
收藏
页码:229 / 237
页数:9
相关论文
共 34 条
[11]  
HOLT JG, 1993, BERGERS MANUAL DETER
[12]   Probiotics in aquaculture [J].
Irianto, A ;
Austin, B .
JOURNAL OF FISH DISEASES, 2002, 25 (11) :633-642
[13]  
Irianto A., 2002, J FISH DIS, V25, P1, DOI DOI 10.1046/J.1365-2761.2002.00375.X
[14]  
Jayanth K, 2001, BULL EUR ASSN FISH P, V21, P12
[15]   Effect of adherent Lactobacillus spp on in vitro adherence of salmonellae to the intestinal epithelial cells of chicken [J].
Jin, LZ ;
Ho, YW ;
Ali, MA ;
Abdullah, N ;
Jalaludin, S .
JOURNAL OF APPLIED BACTERIOLOGY, 1996, 81 (02) :201-206
[16]   Colonization in the fish intestinal tract and production of inhibitory substances in intestinal mucus and faecal extracts by Carnobacterium sp. strain K1 [J].
Joborn, A ;
Olsson, JC ;
Westerdahl, A ;
Conway, PL ;
Kjelleberg, S .
JOURNAL OF FISH DISEASES, 1997, 20 (05) :383-392
[17]   Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli [J].
Lee, YK ;
Puong, KY ;
Ouwehand, AC ;
Salminen, S .
JOURNAL OF MEDICAL MICROBIOLOGY, 2003, 52 (10) :925-930
[18]   THE ADHERENCE OF LACTIC-ACID BACTERIA TO THE COLUMNAR EPITHELIAL-CELLS OF PIGS AND CALVES [J].
MAYRAMAKINEN, A ;
MANNINEN, M ;
GYLLENBERG, H .
JOURNAL OF APPLIED BACTERIOLOGY, 1983, 55 (02) :241-245
[19]   Characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish [J].
Nikoskelainen, S ;
Salminen, S ;
Bylund, G ;
Ouwehand, AC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (06) :2430-2435
[20]  
Olsson JC, 1996, J FISH DIS, V19, P225, DOI 10.1111/j.1365-2761.1996.tb00129.x