Atomic force microscopy investigation of vocal fold collagen

Objectives: Collagen is an important constituent of the vocal fold extracellular matrix and is necessary for providing tensile strength and maintaining tissue geometry. Traditional investigations of vocal fold Collagen using light and electron microscopy do not provide information on the organization and mechanical properties of Collagen in native topographic state. The primary objective of this study was to use Atomic Force Microscopy (AFM) to examine the surface characteristics and organization of Collagen in the deep layer of the lamina propria at nanoscale resolution. Study Design: Experimental in vitro design. Methods: Freshly dissected porcine vocal folds were mounted on AFM discs and imaged under contact and tapping mode to obtain information on topographic distribution of Collagen. Results: AFM imaging of the deep layer of the lamina propria. revealed dense, abundant Collagen fibers with a characteristic banding pattern. The distribution of collagen was heterogeneous, with both hydrophilic and hydrophobic regions within a sample. Conclusions: AFM offers a useful tool to obtain topographic information about biologic samples at nanoscale resolution with minimal sample preparation. Mapping the topography and mechanical properties of vocal fold Collagen is necessary for designing rheologically compatible bioimplants for the treatment of dysphonia I resulting from vocal fold scarring and bowing.