Inhibitory effects of surfactant protein A on surfactant phospholipid hydrolysis by secreted phospholipases A2

被引:47
作者
Chabot, S
Koumanov, K
Lambeau, G
Gelb, MH
Balloy, V
Chignard, M
Whitsett, JA
Touqui, L
机构
[1] Inst Pasteur, INSERM E 336, Unite Def Innee & Inflammat, F-75015 Paris, France
[2] Univ Paris 06, Biochim Lab, CNRS, Unite Rech Asociee, Paris, France
[3] CNRS, Inst Pharmacol Mol & Cellulaire, F-06560 Valbonne, France
[4] Univ Washington, Dept Chem, Seattle, WA 98195 USA
[5] Univ Washington, Dept Biochem, Seattle, WA 98195 USA
[6] Univ Cincinnati, Childrens Hosp, Med Ctr, Div Pulm Biol, Cincinnati, OH 45229 USA
关键词
D O I
10.4049/jimmunol.171.2.995
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Hydrolysis of surfactant phospholipids by secreted phospholipases A(2) (sPLA(2)) contributes to surfactant dysfunction in acute respiratory distress syndrome. The present study demonstrates that sPLA(2)-IIA, sPLA(2)-V, and sPLA(2)-X efficiently hydrolyze surfactant phospholipids in vitro. In contrast, sPLA(2)-IIC, -IID, -IIE, and -IIF have no effect. Since purified surfactant protein A (SP-A) has been shown to inhibit sPLA(2)-IIA activity, we investigated the in vitro effect of SP-A on the other active sPLA(2) and the consequences of sPLA(2)-IIA inhibition by SP-A on surfactant phospholipid hydrolysis. SP-A inhibits sPLA(2)-X activity, but fails to interfere with that of sPLA(2)-V. Moreover, in vitro inhibition of sPLA(2)-IIA-induces surfactant phospholipid hydrolysis correlates with the concentration of SP-A in surfactant. Intratracheal administration of sPLA(2)-IIA to mice causes hydrolysis of surfactant phosphatidylglycerol. Interestingly, such hydrolysis,is significantly higher for SP-A gene-targeted mice, showing the in vivo inhibitory effect of SP-A on sPLA(2)-IIA activity. Administration of sPLA(2)-IIA also induces respiratory distress, which is more pronounced in SP-A gene-targeted mice than in wild-type mice. We conclude that SP-A inhibits sPLA(2) activity, which may play a protective role by maintaining surfactant integrity during lung injury.
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页码:995 / 1000
页数:6
相关论文
共 48 条
[1]   MULTIFUNCTIONAL ACTIVITY OF THE EXTRACELLULAR DOMAIN OF THE M-TYPE (180 KDA) MEMBRANE-RECEPTOR FOR SECRETORY PHOSPHOLIPASES A(2) [J].
ANCIAN, P ;
LAMBEAU, G ;
LAZDUNSKI, M .
BIOCHEMISTRY, 1995, 34 (40) :13146-13151
[2]   Generation of lyso-phospholipids from surfactant in acute lung injury is mediated by type-II phospholipase A2 and inhibited by a direct surfactant protein A-phospholipase A2 protein interaction [J].
Arbibe, L ;
Koumanov, K ;
Vial, D ;
Rougeot, C ;
Faure, G ;
Havet, N ;
Longacre, S ;
Vargaftig, BB ;
Béréziat, G ;
Voelker, DR ;
Wolf, C ;
Touqui, L .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 102 (06) :1152-1160
[3]   Damage to surfactant-specific protein in acute respiratory distress syndrome [J].
Baker, CS ;
Evans, TW ;
Randle, BJ ;
Haslam, PL .
LANCET, 1999, 353 (9160) :1232-1237
[4]   PHYSICAL-PROPERTIES OF AN EFFECTIVE LUNG SURFACTANT [J].
BANGHAM, AD ;
MORLEY, CJ ;
PHILLIPS, MC .
BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 573 (03) :552-556
[5]   Interfacial enzymology:: The secreted phospholipase A2-paradigm [J].
Berg, OG ;
Gelb, MH ;
Tsai, MD ;
Jain, MK .
CHEMICAL REVIEWS, 2001, 101 (09) :2613-2653
[6]   Exogenously added human group X secreted phospholipase A2 but not the group IB, IIA, and V enzymes efficiently release arachidonic acid from adherent mammalian cells [J].
Bezzine, S ;
Koduri, RS ;
Valentin, E ;
Murakami, M ;
Kudo, I ;
Ghomashchi, F ;
Sadilek, M ;
Lambeau, G ;
Gelb, MH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (05) :3179-3191
[7]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[8]  
BOTTCHER CJF, 1961, ANAL CHIM ACTA, V24, P203
[9]   Both group IB and group IIA secreted phospholipases A2 are natural ligands of the mouse 180-kDa M-type receptor [J].
Cupillard, L ;
Mulherkar, R ;
Gomez, N ;
Kadam, S ;
Valentin, E ;
Lazdunski, M ;
Lambeau, G .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (11) :7043-7051
[10]   Cloning, chromosomal mapping, and expression of a novel human secretory phospholipase A(2) [J].
Cupillard, L ;
Koumanov, K ;
Mattei, MG ;
Lazdunski, M ;
Lambeau, G .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (25) :15745-15752