Strategy for Rapid Identification and Antibiotic Susceptibility Testing of Gram-Negative Bacteria Directly Recovered from Positive Blood Cultures Using the Bruker MALDI Biotyper and the BD Phoenix System

被引:53
作者
Wimmer, Jana L. [1 ]
Long, S. Wesley [1 ,2 ]
Cernoch, Patricia [1 ]
Land, Geoffrey A. [1 ]
Davis, James R. [1 ]
Musser, James M. [1 ,2 ]
Olsen, Randall J. [1 ,2 ]
机构
[1] Methodist Hosp, Dept Pathol & Genom Med, Houston, TX 77030 USA
[2] Methodist Hosp, Res Inst, Ctr Mol & Translat Human Infect Dis Res, Houston, TX 77030 USA
关键词
DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; DIRECT INOCULATION; CLINICAL IMPACT; BOTTLES; VITEK-2;
D O I
10.1128/JCM.00409-12
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Decreasing the time to species identification and antibiotic susceptibility determination of strains recovered from patients with bacteremia significantly decreases morbidity and mortality. Herein, we validated a method to identify Gram-negative bacteria directly from positive blood culture medium using the Bruker MALDI Biotyper and to rapidly perform susceptibility testing using the BD Phoenix.
引用
收藏
页码:2452 / 2454
页数:3
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