Strategy for Rapid Identification and Antibiotic Susceptibility Testing of Gram-Negative Bacteria Directly Recovered from Positive Blood Cultures Using the Bruker MALDI Biotyper and the BD Phoenix System

被引：53

作者：

Wimmer, Jana L. ^{[1
]}

Long, S. Wesley ^{[1
,2
]}

Cernoch, Patricia ^{[1
]}

Land, Geoffrey A. ^{[1
]}

Davis, James R. ^{[1
]}

Musser, James M. ^{[1
,2
]}

Olsen, Randall J. ^{[1
,2
]}

机构：

[1] Methodist Hosp, Dept Pathol & Genom Med, Houston, TX 77030 USA

[2] Methodist Hosp, Res Inst, Ctr Mol & Translat Human Infect Dis Res, Houston, TX 77030 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 07期

关键词：

DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; DIRECT INOCULATION; CLINICAL IMPACT; BOTTLES; VITEK-2;

D O I：

10.1128/JCM.00409-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Decreasing the time to species identification and antibiotic susceptibility determination of strains recovered from patients with bacteremia significantly decreases morbidity and mortality. Herein, we validated a method to identify Gram-negative bacteria directly from positive blood culture medium using the Bruker MALDI Biotyper and to rapidly perform susceptibility testing using the BD Phoenix.

引用

页码：2452 / 2454

页数：3

共 37 条

[1] Rapid Identification and Antimicrobial Susceptibility Testing Directly from Blood Cultures of Gram-negative and Gram-positive Isolates
Kong, Haishen
Zhang, Shujin
Chen, Xiao
Zhang, Weili
Yang, Qing
Fu, Yajie
Wang, Yiyin
Li, Xuefen
CLINICAL LABORATORY, 2013, 59 (11-12) : 1305 - 1310
[2] Comparative evaluation of the Vitek-2 Compact and Phoenix systems for rapid identification and antibiotic susceptibility testing directly from blood cultures of Gram-negative and Gram-positive isolates
Gherardi, Giovanni
Angeletti, Silvia
Panitti, Miriam
Pompilio, Arianna
Di Bonaventura, Giovanni
Crea, Francesca
Avola, Alessandra
Fico, Laura
Palazzo, Carlo
Sapia, Genoveffa Francesca
Visaggio, Daniela
Dicuonzo, Giordano
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 72 (01) : 20 - 31
[3] Identification and antimicrobial susceptibility testing of Gram-positive and Gram-negative bacteria from positive blood cultures using the Accelerate Pheno™ system
Ullberg, Mans
Ozenci, Volkan
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2020, 39 (01) : 139 - 149
[4] Rapid and reliable identification of Gram-negative bacteria and Gram-positive cocci by deposition of bacteria harvested from blood cultures onto the MALDI-TOF plate
Barnini, Simona
Ghelardi, Emilia
Brucculeri, Veronica
Morici, Paola
Lupetti, Antonella
BMC MICROBIOLOGY, 2015, 15
[5] Saponin promotes rapid identification and antimicrobial susceptibility profiling of Gram-positive and Gram-negative bacteria in blood cultures with the Vitek 2 system
Lupetti, A.
Barnini, S.
Morici, P.
Ghelardi, E.
Nibbering, P. H.
Campa, M.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2013, 32 (04) : 493 - 502
[6] Direct inoculation method using BacT/ALERT 3D and BD Phoenix System allows rapid and accurate identification and susceptibility testing for both Gram-positive cocci and Gram-negative rods in aerobic blood cultures
Yonetani, Shota
Okazaki, Mitsuhiro
Araki, Koji
Makino, Hiroshi
Fukugawa, Yoko
Okuyama, Takahiro
Ohnishi, Hiroaki
Watanabe, Takashi
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 2012, 73 (02) : 129 - 134
[7] Rapid identification and antimicrobial susceptibility testing of Gram-negative rod on positive blood cultures using MicroScan panels
Infante, A.
de la Tabla, V. Ortiz
Martin, C.
Gazquez, G.
Bunuel, F.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2021, 40 (01) : 151 - 157
[8] Microbial identification and automated antibiotic susceptibility testing directly from positive blood cultures using MALDI-TOF MS and VITEK 2
Wattal, C.
Oberoi, J. K.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2016, 35 (01) : 75 - 82
[9] Anidulafungin Susceptibility Testing of Candida glabrata Isolates from Blood Cultures by the MALDI Biotyper Antibiotic (Antifungal) Susceptibility Test Rapid Assay
Vatanshenassan, Mansoureh
Arastehfar, Amir
Boekhout, Teun
Berman, Judith
Lass-Floerl, Cornelia
Sparbier, Katrin
Kostrzewa, Markus
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2019, 63 (09)
[10] Rapid and reliable identification of Gram-negative bacteria and Gram-positive cocci by deposition of bacteria harvested from blood cultures onto the MALDI-TOF plate
Simona Barnini
Emilia Ghelardi
Veronica Brucculeri
Paola Morici
Antonella Lupetti
BMC Microbiology, 15

← 1 2 3 4 →