Continuous assessment of human spermatozoa viability during cryopreservation

被引:0
|
作者
Mohammad, SN [1 ]
Barratt, CL [1 ]
Cooke, ID [1 ]
Moore, HD [1 ]
机构
[1] UNIV SHEFFIELD,JESSOP HOSP WOMEN,DEPT OBSTET & GYNAECOL,SHEFFIELD S3 7RE,S YORKSHIRE,ENGLAND
来源
JOURNAL OF ANDROLOGY | 1997年 / 18卷 / 01期
关键词
sperm; cryomicroscopy; fluorescent viability probes; cryosurvival;
D O I
暂无
中图分类号
R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
摘要
Cryomicroscopy has enabled direct observation of freezing and thawing of human spermatozoa. When used with a fluorescent viability kit, sperm membrane damage was not apparent down to temperatures of -5 degrees C, but significant damage occurred after thawing (55% of spermatozoa had damaged membranes). Semen samples were cooled or frozen to temperatures (at decrements of 10 degrees C) from 0 degrees C to -110 degrees C. At all these temperatures the proportion of live to membrane-damaged cells remained constant. Samples held at temperatures above -30 degrees C were not adversely affected. Below -30 degrees C there was a gradual increase in the proportion of membrane-damaged cells on thaw and a decrease in the number of live cells recovering motility. At temperatures between -50 degrees C and -60 degrees C there was an equal proportion of live motile, immotile, and membrane-damaged cells. It is concluded that some irreversible damage to spermatozoa was a result of freezing processes in cells frozen to -30 degrees C or less, but most of the cryodamage was incurred during thawing, possibly due to recrystallization.
引用
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页码:43 / 50
页数:8
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