Sphingobacterium tabacisoli sp nov., isolated from a tobacco field soil sample

A Gram-staining-negative, non-motile and rod-shaped bacterium, designated strain h337(T), was isolated from an arable soil sample of a tobacco field in Kunming, south-west China. The cells showed oxidase-positive and catalase-positive reactions. Growth was observed at 10-35 degrees C, at pH 6.0-9.0 and in the presence of up to 3% (w/v) NaCl, with optimal growth at 30 degrees C, pH 7.0 and with 1-2% (w/v) NaCl. The predominant isoprenoid quinone was MK-7. The major fatty acids were identified as iso-C-15:0, iso-C-17:0 3-OH, summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c) and summed feature 4 (iso-C-17:1 I and/or anteiso-C-17:1 B). The cellular polar lipids contained phosphatidylethanolamine, sphingophospholipid, four unidentified phospholipids, five unidentified lipids and three unidentified aminophospholipids. The genomic DNA G+C content was 41.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain h337(T) should be assigned to the genus Sphingobacterium. 16S rRNA gene sequence similarity analysis showed that strain h337(T) was most closely related to 'Sphingobacterium yamdrokense' 3-0-1 (98.8 %) and Sphingobacterium yanglingense CCNWSP36-1(T) (98.5 %) and shared less than 97% similarity with other species of the genus Sphingobacterium. DNA-DNA hybridization data indicated that the isolate represented a novel genomic species belonging to the genus Sphingobacterium. The characteristics determined in this polyphasic taxonomic study indicated that strain h337(T) represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium tabacisoli sp. nov. (type strain h337(T)=KCTC 52298(T)=CCTCC AB 2017155(T)) is proposed.