Antimicrobial efficacy of a polymeric chlorhexidine release device using in vitro model of Enterococcus faecalis dentinal tubule infection

被引：36

作者：

Lee, Yoon ^{[2
]}

Han, Seung Hyun ^{[3
,4
]}

Hong, Sang-Hee

Lee, Jin-Kyung ^{[1
]}

Ji, Hye ^{[1
]}

Kum, Kee-Yeon ^{[1
]}

机构：

[1] Seoul Natl Univ, Sch Dent, Inst Dent Res, Dept Conservat Dent, Seoul, South Korea

[2] Wonkwang Univ, Dent Hosp, Dept Conservat Dent, Taejon, South Korea

[3] Seoul Natl Univ, Dept Oral Microbiol & Immunol, Dent Res Inst, Seoul, South Korea

[4] Seoul Natl Univ, BK21 Program, Sch Dent, Seoul, South Korea

来源：

JOURNAL OF ENDODONTICS | 2008年 / 34卷 / 07期

基金：

新加坡国家研究基金会;

关键词：

antimicrobial efficacy; cylindrical dentin block; Enterococcus faecalis; optical density; polymeric chlorhexidine-controlled release device;

D O I：

10.1016/j.joen.2008.04.008

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

This study aimed to assess the antimicrobial efficacy of a novel polymeric chlorhexidine-control led release device as an intracanal medicament. One hundred cylindrical dentin blocks prepared from human single-rooted teeth were inoculated with Enterococcus faecalis for 3 weeks. The intracanal medicaments tested were calcium hydroxide, a polymeric chlorhexidine-control led release device (PCRD), a polymeric controlled release device without chlorhexidine (CHx), 0.2% CHx solution, and sterile saline. Dentin samples (at 200-mu m and 400-mu m depths) were collected from the medicated canal lumens after 1 week of medication with sterile LightSpeed files and placed in growth medium. Bacterial growth was assessed spectrophotometrically by analysis of optical density (OD) after 24 hours of incubation. The OD values at both depths were significantly lower in the PCRD group than in the other experimental groups (P <.001). These results indicate that a PCRD can be an effective intracanal medicament against E. faecalis.

引用

页码：855 / 858

页数：4

共 35 条

[1] Antimicrobial action of calcium hydroxide, chlorhexid.ine and their combination on endodontic pathogens [J].

Ballal, V. ;

Kundabala, M. ;

Acharya, S. ;

Ballal, M. .

AUSTRALIAN DENTAL JOURNAL, 2007, 52 (02) :118-121

[2] Substantive antimicrobial activity in chlorhexidine-treated human root dentin [J].

Basrani, B ;

Santos, JM ;

Tjäderhane, L ;

Grad, H ;

Gorduysus, O ;

Huang, JF ;

Lawrence, HP ;

Friedman, S .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2002, 94 (02) :240-245

[3]

BYSTROM A, 1985, Endodontics and Dental Traumatology, V1, P170

[4]

BYSTROM A, 1981, SCAND J DENT RES, V89, P321

[5] THE ROLE OF INTRACANAL MEDICATION IN ROOT-CANAL TREATMENT [J].

CHONG, BS ;

FORD, TRP .

INTERNATIONAL ENDODONTIC JOURNAL, 1992, 25 (02) :97-106

[6]

DAVIES A, 1973, J PERIODONTAL RES, P68

[7] Mechanisms involved in the resistance of Enterococcus faecalis to calcium hydroxide [J].

Evans, M ;

Davies, JK ;

Sundqvist, G ;

Figdor, D .

INTERNATIONAL ENDODONTIC JOURNAL, 2002, 35 (03) :221-228

[8]

Garcez Aguinaldo Silva, 2007, Braz. Dent. J., V18, P202

[9] Effectiveness of 2% chlorhexidine gel and calcium hydroxide against Enterococcus faecalis in bovine root dentine in vitro [J].

Gomes, BPFA ;

Souza, SFC ;

Ferraz, CCR ;

Teixeira, FB ;

Zaia, AA ;

Valdrighi, L ;

Souza, FJ .

INTERNATIONAL ENDODONTIC JOURNAL, 2003, 36 (04) :267-275

[10] INVITRO INFECTION AND DISINFECTION OF DENTINAL TUBULES [J].

HAAPASALO, M ;

ORSTAVIK, D .

JOURNAL OF DENTAL RESEARCH, 1987, 66 (08) :1375-1379

← 1 2 3 4 →