Vertical-flow constructed wetland based on pyrite intensification: Mixotrophic denitrification performance and mechanism

被引：73

作者：

Chu, Yifan ^{[1
,2
]}

Liu, Wei ^{[1
,2
]}

Tan, Qiyang ^{[1
,2
]}

Yang, Lingli ^{[1
,2
]}

Chen, Jinmei ^{[1
,2
]}

Ma, Lin ^{[3
]}

Zhang, Yi ^{[1
]}

Wu, Zhenbin ^{[1
]}

He, Feng ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

[3] Chinese Acad Sci, CAS Key Lab Aquat Bot & Watershed Ecol, Wuhan Bot Garden, Wuhan 430072, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 347卷

基金：

中国国家自然科学基金;

关键词：

Pyrite; Vertical-flow constructed wetland; Autotrophic denitrification; Mixotrophic denitrification; Microbial community succession; AUTOTROPHIC DENITRIFICATION; PHOSPHORUS REMOVAL; NITROGEN REMOVAL; BACTERIAL COMMUNITY; NITRATE REDUCTION; FE(II) OXIDATION; ELECTRON-DONORS; WASTE-WATER; GROUNDWATER; PARAMETERS;

D O I：

10.1016/j.biortech.2022.126710

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Deep nitrogen removal from low-carbon wastewater is a pressing water treatment challenge as of yet. Eight sets of vertical-flow constructed wetland (VFCW) intensified by pyrite were designed and applied to treat with low C/ N ratio wastewater in this research. The results showed that the addition of pyrite (100% added) significantly promoted TN removal with an efficiency higher than 27.05% under low C/N ratio conditions, indicating that mixotrophic denitrification was achieved in VFCW. Microbial analysis showed that the community structure and diversity of microorganisms were changed significantly, and the growth of autotrophic (Thiobacillus) and heterotrophic bacteria (Thauera) concomitantly enhanced. It is recommended that the addition amount of pyrite is 75% of the wetland volume, meantime, mixing evenly with 25% high porosity substrate (such as activated carbon, volcanic stone, etc.), which could enhance the effective adhesion of microorganisms and their contact area with pyrite, ultimately improve the denitrification capacity of the VFCW.

引用

页数：10

共 50 条

[1] Development of enhanced SNAD process in a down-flow packed bed reactor for removal of higher concentrations of NH4-N and COD [J].

Anjali, G. ;

Sabumon, P. C. .

JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, 2015, 3 (02) :1009-1017

[2] Characterisation of the soil bacterial community structure and composition of natural and constructed wetlands [J].

Ansola, Gemma ;

Arroyo, Paula ;

Saenz de Miera, Luis E. .

SCIENCE OF THE TOTAL ENVIRONMENT, 2014, 473 :63-71

[3] Genorne-enabled studies of anaerobic, nitrate-dependent iron oxidation in the chemolithoautotrophic bacterium Thiobacillus denitrificans [J].

Beller, Harry R. ;

Zhou, Peng ;

Legler, Tina C. ;

Chakicherla, Anu ;

Kane, Staci ;

Letain, Tracy E. ;

O'Day, Peggy A. .

FRONTIERS IN MICROBIOLOGY, 2013, 4

[4] Anaerobic, Nitrate-Dependent Oxidation of Pyrite Nanoparticles by Thiobacillus denitrificans [J].

Bosch, Julian ;

Lee, Keun-Young ;

Jordan, Guntram ;

Kim, Kyoung-Woong ;

Meckenstock, Rainer U. .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2012, 46 (04) :2095-2101

[5] Enrichment of Autotrophic Denitrifiers From Anaerobic Sludge Using Sulfurous Electron Donors [J].

Carboni, M. F. ;

Florentino, A. P. ;

Costa, R. B. ;

Zhan, X. ;

Lens, P. N. L. .

FRONTIERS IN MICROBIOLOGY, 2021, 12

[6] Performance of experimental horizontal subsurface flow constructed wetlands fed with dissolved or particulate organic matter [J].

Caselles-Osorio, Aracelly ;

Garcia, Joan .

WATER RESEARCH, 2006, 40 (19) :3603-3611

[7] Induction of Nitrate-Dependent Fe(II) Oxidation by Fe(II) in Dechloromonas sp Strain UWNR4 and Acidovorax sp Strain 2AN [J].

Chakraborty, Anirban ;

Picardal, Flynn .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2013, 79 (02) :748-752

[8] Study of pyrite based autotrophic denitrification system for low-carbon source stormwater treatment [J].

Chen, Yifan ;

Shao, Zhiyu ;

Kong, Zheng ;

Gu, Li ;

Fang, Junhua ;

Chai, Hongxiang .

JOURNAL OF WATER PROCESS ENGINEERING, 2020, 37

[9] Simultaneous denitrification, phosphorus recovery and low sulfate production in a recirculated pyrite-packed biofilter (RPPB) [J].

Di Capua, Francesco ;

Mascolo, Maria Cristina ;

Pirozzi, Francesco ;

Esposito, Giovanni .

CHEMOSPHERE, 2020, 255

[10] Preparation of a novel water-soluble organosilane coating and its performance for inhibition of pyrite oxidation to control acid mine drainage at the source [J].

Dong, Yingbo ;

Zeng, Weihong ;

Lin, Hai ;

He, Yinhai .

APPLIED SURFACE SCIENCE, 2020, 531

← 1 2 3 4 5 →