Biosynthesis of an anti- tuberculosis sesterterpenoid asperterpenoid A

被引：32

作者：

Huang, Jia-Hua ^{[1
]}

Lv, Jian-Ming ^{[1
]}

Wang, Qiao-Zhen ^{[1
]}

Zou, Jian ^{[1
]}

Lu, Yong-Jun ^{[2
]}

Wang, Qing-Lin ^{[2
]}

Chen, Dong-Ni ^{[3
]}

Yao, Xin-Sheng ^{[1
]}

Gao, Hao ^{[1
]}

Hu, Dan ^{[1
]}

机构：

[1] Jinan Univ, Guangdong Prov Key Lab Pharmacodynam Constiuents, Coll Pharm, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Sch Life Sci, Guangzhou 510275, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Sch Marine Sci, Guangzhou 510275, Guangdong, Peoples R China

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2019年 / 17卷 / 02期

基金：

中国国家自然科学基金;

关键词：

GENOME MINING APPROACH; TERPENE SYNTHASES; DISCOVERY;

D O I：

10.1039/c8ob02832j

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A putative three-gene cluster for asperterpenoid A was identified. Step-wise reconstitution of this gene cluster in Aspergillus oryzae reveals that astC encodes a sesterterpene cyclase to synthesize preasperterpenoid A, which is dually oxidized by a P450 enzyme AstB to give asperterpenoid A along with a minor product asperterpenoid B, and asperterpenoid A is further oxidized by another P450 eznyme AstA to afford a new sesterterpenoid asperterpenoid C. Unexpectedly, asperterpenoids A and B, but not the final product asperterpenoid C, exhibit potent inhibitory activity against Mycobacterium tuberculosis protein tyrosine phosphatase B with IC50 values of 3-6 M.

引用

页码：248 / 251

页数：4

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