Effect of Pentoxifylline on Renal Ischemic Reperfusion

Ischemic/reperfusion induced cell death and inflammatory reaction (acute renal failure) is a common clinical problem associated with acute renal failure and renal transplantation. In this study, the effects of pentoxifylline on the attenuation of an ischemia reperfusion injury and inflammatory reaction were examined. Twelve adult mixed breed dogs of both sexes, weighing 10-20 kg were chosen and then the dogs were assigned randomly into control and treatment groups (n = 6). Celiotomy was performed by ventral midline incision. The left kidney was isolated and then both the renal artery and vein were clamped. After 60 min of warm ischemia, the vessels were undamped and followed by 72 h of reperfusion while the right kidney was removed. Blood samples were collected before ischemia and at 24, 48 and 72 h after ischemia for determination of serum creatinine and BUN level. After 72 h of ischemia-reperfusion tissue samples from the left kidney were taken for histopathology examination. The treatment group showed lower creatinine, BUN (p<0.001 each), cell death (p<0.05 each) and of renal inflammatory reactions than did the control group.