A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71

被引：353

作者：

Wang, Xiangxi ^{[2
]}

Peng, Wei ^{[2
]}

Ren, Jingshan ^{[1
]}

Hu, Zhongyu ^{[3
]}

Xu, Jiwei ^{[2
]}

Lou, Zhiyong ^{[4
]}

Li, Xumei ^{[2
]}

Yin, Weidong ^{[3
]}

Shen, Xinliang ^{[3
]}

Porta, Claudine ^{[1
]}

Walter, Thomas S. ^{[1
]}

Evans, Gwyndaf ^{[5
]}

Axford, Danny ^{[5
]}

Owens, Robin ^{[5
]}

Rowlands, David J. ^{[6
,7
]}

Wang, Junzhi ^{[3
]}

Stuart, David I. ^{[1
,5
]}

Fry, Elizabeth E. ^{[1
]}

Rao, Zihe ^{[2
,4
]}

机构：

[1] Univ Oxford, Div Struct Biol, Oxford, England

[2] Chinese Acad Sci, Inst Biophys, Natl Lab Macromol, Beijing 100080, Peoples R China

[3] Natl Inst Food & Drug Control, Beijing, Peoples R China

[4] Tsinghua Univ, Sch Med, Struct Biol Lab, Beijing 100084, Peoples R China

[5] Diamond Light Sources, Didcot, Oxon, England

[6] Univ Leeds, Inst Mol & Cellular Biol, Fac Biol Sci, Leeds, W Yorkshire, England

[7] Univ Leeds, Astbury Ctr Struct Mol Biol, Fac Biol Sci, Leeds, W Yorkshire, England

来源：

NATURE STRUCTURAL & MOLECULAR BIOLOGY | 2012年 / 19卷 / 04期

基金：

英国惠康基金; 英国医学研究理事会;

关键词：

CRYSTAL-STRUCTURE; CELLULAR RECEPTOR; EXTERNALIZED POLYPEPTIDE; MATURATION CLEAVAGE; HUMAN RHINOVIRUS-14; CAPSID PROTEIN; POLIOVIRUS; VIRUS; IDENTIFICATION; RESOLUTION;

D O I：

10.1038/nsmb.2255

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Enterovirus 71 (EV71) is a major agent of hand, foot and mouth disease in children that can cause severe central nervous system disease and death. No vaccine or antiviral therapy is available. High-resolution structural analysis of the mature virus and natural empty particles shows that the mature virus is structurally similar to other enteroviruses. In contrast, the empty particles are markedly expanded and resemble elusive enterovirus-uncoating intermediates not previously characterized in atomic detail. Hydrophobic pockets in the EV71 capsid are collapsed in this expanded particle, providing a detailed explanation of the mechanism for receptor-binding triggered virus uncoating. These structures provide a model for enterovirus uncoating in which the VP1 GH loop acts as an adaptor-sensor for cellular receptor attachment, converting heterologous inputs to a generic uncoating mechanism, highlighting new opportunities for therapeutic intervention.

引用

页码：424 / 429

页数：6

共 51 条

[1] AMINO-ACID SUBSTITUTIONS IN THE POLIOVIRUS MATURATION CLEAVAGE SITE AFFECT ASSEMBLY AND RESULT IN ACCUMULATION OF PROVIRIONS [J].

ANSARDI, DC ;

MORROW, CD .

JOURNAL OF VIROLOGY, 1995, 69 (03) :1540-1547

[2] ROLE AND MECHANISM OF THE MATURATION CLEAVAGE OF VPO IN POLIOVIRUS ASSEMBLY - STRUCTURE OF THE EMPTY CAPSID ASSEMBLY INTERMEDIATE AT 2.9-ANGSTROM RESOLUTION [J].

BASAVAPPA, R ;

SYED, R ;

FLORE, O ;

ICENOGLE, JP ;

FILMAN, DJ ;

HOGLE, JM .

PROTEIN SCIENCE, 1994, 3 (10) :1651-1669

[3] Molecular tectonic model of virus structural transitions: the putative cell entry states of poliovirus [J].

Belnap, DM ;

Filman, DJ ;

Trus, BL ;

Cheng, NQ ;

Booy, FP ;

Conway, JF ;

Curry, S ;

Hiremath, CN ;

Tsang, SK ;

Steven, AC ;

Hogle, JM .

JOURNAL OF VIROLOGY, 2000, 74 (03) :1342-1354

[4] DETECTION BY MONOCLONAL-ANTIBODIES OF AN ANTIGENIC DETERMINANT CRITICAL FOR POLIOVIRUS NEUTRALIZATION PRESENT ON VP1 AND ON HEAT-INACTIVATED VIRIONS [J].

BLONDEL, B ;

AKACEM, O ;

CRAINIC, R ;

COUILLIN, P ;

HORODNICEANU, F .

VIROLOGY, 1983, 126 (02) :707-710

[5] Poliovirus RNA Is Released from the Capsid near a Twofold Symmetry Axis [J].

Bostina, Mihnea ;

Levy, Hazel ;

Filman, David J. ;

Hogle, James M. .

JOURNAL OF VIROLOGY, 2011, 85 (02) :776-783

[6] Complete nucleotide sequence of enterovirus 71 is distinct from poliovirus [J].

Brown, BA ;

Pallansch, MA .

VIRUS RESEARCH, 1995, 39 (2-3) :195-205

[7] The structure of the poliovirus 135S cell entry intermediate at 10-Angstrom resolution reveals the location of an externalized polypeptide that binds to membranes [J].

Bubeck, D ;

Filman, DJ ;

Cheng, NQ ;

Steven, AC ;

Hogle, JM ;

Belnap, DM .

JOURNAL OF VIROLOGY, 2005, 79 (12) :7745-7755

[8] SPECIFIC ALTERATIONS OF COXSACKIEVIRUS B3 ELUTED FROM HELA CELLS [J].

CROWELL, RL ;

PHILIPSON, L .

JOURNAL OF VIROLOGY, 1971, 8 (04) :509-+

[9] Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: The structure of empty capsids of foot-and-mouth disease virus [J].

Curry, S ;

Fry, E ;

Blakemore, W ;

AbuGhazaleh, R ;

Jackson, T ;

King, A ;

Lea, S ;

Newman, J ;

Stuart, D .

JOURNAL OF VIROLOGY, 1997, 71 (12) :9743-9752

[10] Recombinant VP4 of human rhinovirus induces permeability in model membranes [J].

Davis, Matthew P. ;

Bottley, Graham ;

Beales, Lucy P. ;

Killington, Richard A. ;

Rowlands, David J. ;

Tuthill, Tobias J. .

JOURNAL OF VIROLOGY, 2008, 82 (08) :4169-4174

← 1 2 3 4 5 6 →