Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants in peanut (Arachis hypogaea L.)

被引：29

作者：

Geng, Lili ^{[1
]}

Niu, Lihong ^{[1
]}

Gresshoff, Peter M. ^{[2
]}

Shu, Changlong ^{[1
]}

Song, Fuping ^{[1
]}

Huang, Dafang ^{[3
]}

Zhang, Jie ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

[2] Univ Queensland, ARC Ctr Excellence Integrat Legume Res, Brisbane, Qld 4072, Australia

[3] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2012年 / 109卷 / 03期

基金：

澳大利亚研究理事会;

关键词：

Agrobacterium rhizogenes; Peanut; Transformation; Microinjection; cry8Ea1; gene; MEDIATED TRANSFORMATION; HAIRY ROOTS; REGENERATION; GENE; SYSTEM; TUMEFACIENS; INDUCTION; SYMBIOSIS;

D O I：

10.1007/s11240-012-0113-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Recalcitrance of most large-seeded legumes, such as peanut, to regeneration and genetic transformation has hampered studies on gene function and efforts for genetic improvement. Agrobacterium rhizogenes-mediated transformation provides a system for rapid and efficient transformation of plant tissues. In this study, embryonic axes along with cotyledons of peanut were injected with a suspension culture of A. rhizogenes using microliter syringes. The influence of several factors such as plant genotype, A. rhizogenes culture stage, co-culture period of A. rhizogenes, and acetosyringone concentration in the co-cultivationmedium have been evaluated. It is found that A. rhizogenes-mediated transformation of peanut is genotype-independent. Up to 61% transformation was recorded when embryonic axes were co-cultivated with 5 x 10(7) A. rhizogenes cells from logarithmic phase for 2 days on co-culture medium containing 50 mu mol l(-1) acetosyringone. Composite plants with transgenic roots were harvested after 45 days of treatment. Furthermore, this method was applied to assess the insecticidal activity of a synthetic cry8Ea1 gene against Holotrichia parallela in transgenic roots of peanut.

引用

页码：491 / 500

页数：10

共 44 条

[1] Morphological alterations and root nodule formation in Agrobacterium rhizogenas-mediated transgenic hairy roots of peanut (Arachis hypogaea L.) [J].