Production of L-phenylacetyl carbinol by immobilized cells of Saccharomyces cerevisiae

被引：15

作者：

Mandwal, AK ^{[1
]}

Tripathi, CKM ^{[1
]}

Trivedi, PD ^{[1
]}

Joshi, AK ^{[1
]}

Agarwal, SC ^{[1
]}

Bihari, V ^{[1
]}

机构：

[1] Cent Drug Res Inst, Div Fermentat Technol, Lucknow 226001, Uttar Pradesh, India

来源：

BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 03期

关键词：

benzaldehyde; biotransformation; immobilized biocatalysts; reactor;

D O I：

10.1023/B:BILE.0000013718.88453.f0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Conversion of benzaldehyde to L-phenylacetyl carbinol (L-PAC) was achieved with immobilized, growing cells of Saccharomyces cerevisiae in different reactors. Product formation increased (31%) with the subsequent initial reuses of the entrapped cells. Biomass production and PAC formation depleted ( 40 and 57%, respectively) after 4 - 5 continuous growth and biotransformation cycles. With the regeneration of the biocatalysts, catalytic activity of the cells was resumed. The highest yields were in a stirred tank reactor ( 29 g PAC) from 77 g benzeldehyde with 14 repeated uses of entrapped cells.

引用

页码：217 / 221

页数：5

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