Shear-induced platelet activation and adhesion on human pulmonary artery endothelial cells seeded onto hydrophilic polymers

被引:0
作者
Sagnella, S
Kwok, J
Marchant, RE
Kottke-Marchant, K [1 ]
机构
[1] Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA
[2] Cleveland Clin Fdn, Dept Clin Pathol, Cleveland, OH 44195 USA
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 2001年 / 57卷 / 03期
关键词
endothelial cell; plasma polymer; platelet activation; platelet adhesion; shear; thromboresistance; flow cytometry; thrombosis;
D O I
10.1002/1097-4636(20011205)57:3<419::AID-JBM1185>3.0.CO;2-I
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We evaluated platelet activation and adhesion on two plasma polymerized surfaces, N-vinyl pyrrolidone (NVP) and gamma -butyro lactone (GBL), which have been shown previously to promote endothelial cell growth and adhesion as well as fibronectin-coated glass (1 mug/cm(2)) coverslips. Human pulmonary artery endothelial cells were seeded onto coverslips at a low density (similar to 20,000 cells/cm(2)) and grown to confluence (3-5 days). The materials, both with and without ECs, were then exposed to a shear rate of 400 in a closed loop recirculating flow system containing human platelet-rich plasma. Plasma samples were taken at 0, 5, 15, 30, and 60 min and analyzed for platelet and coagulation activation. The coverslips were examined for EC coverage and Platelet adherence. EC retention over a 1-h perio was approximately 75% for all three materials. All three materials without ECs were highly platelet activating having similar P-selectin expression, platelet factor 4 (PF4) release, mepacrine uptake, and microparticle production. Both microparticle production and platelet adhesion were significantly lower in EC-seeded materials. Dense granule and PF4 release were both slightly diminished in all three materials seeded with ECs. P-selectin expression was reduced slightly for GBL, but remained the same for the other two materials. The EC-seeded materials displayed favorable characteristics with respect to platelet activation and adhesion; however, they still demonstrated some thrombogenic tendencies due to EC loss and exposure of the underlying substrate. Therefore, both EC coverage and EC hemostatic function are important factors in determining the thromboresistance of an EC-seeded surface. (C) 2001 John Wiley & Sons, Inc.
引用
收藏
页码:419 / 431
页数:13
相关论文
共 46 条
[1]  
ABRAMS C, 1991, THROMB HAEMOSTASIS, V65, P467
[2]   Endothelial cell differentiation into capillary structures by copolymer surfaces with phenylboronic acid groups [J].
Aoki, T ;
Nagao, Y ;
Terada, E ;
Sanui, K ;
Ogata, N ;
Yamada, N ;
Sakurai, Y ;
Kataoka, K ;
Okano, T .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 1995, 7 (07) :539-550
[3]  
BEARN PE, 1994, J R COLL SURG, V36, P1
[4]  
BERGSTROM K, 1994, J BIOMAT SCI-POLYM E, V6, P123
[5]   PLATELET-ADHESION TO FIBRONECTIN IN FLOW - DEPENDENCE ON SURFACE CONCENTRATION AND SHEAR RATE, ROLE OF PLATELET MEMBRANE-GLYCOPROTEINS GP IIB/IIIA AND VLA-5, AND INHIBITION BY HEPARIN [J].
BEUMER, S ;
IJSSELDIJK, MJW ;
DEGROOT, PG ;
SIXMA, JJ .
BLOOD, 1994, 84 (11) :3724-3733
[6]   PLATELET-ADHESION TO FIBRONECTIN IN FLOW - THE IMPORTANCE OF VON-WILLEBRAND-FACTOR AND GLYCOPROTEIN IB [J].
BEUMER, S ;
HEIJNEN, HFG ;
IJSSELDIJK, MJW ;
ORLANDO, E ;
DEGROOT, PG ;
SIXMA, JJ .
BLOOD, 1995, 86 (09) :3452-3460
[7]   PEO ENHANCEMENT OF PLATELET DEPOSITION, FIBRINOGEN DEPOSITION, AND COMPLEMENT C3 ACTIVATION [J].
CHAIKOF, EL ;
MERRILL, EW ;
CALLOW, AD ;
CONNOLLY, RJ ;
VERDON, SL ;
RAMBERG, K .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1992, 26 (09) :1163-1168
[8]  
DACHARYPRIGENT J, 1993, BLOOD, V81, P2554
[9]  
DAHLBACK B, 1992, BIOCHEMISTRY-US, V31, P12768
[10]   FLOW-MEDIATED ENDOTHELIAL MECHANOTRANSDUCTION [J].
DAVIES, PF .
PHYSIOLOGICAL REVIEWS, 1995, 75 (03) :519-560