HIGH HATCHING RATES AFTER CRYOPRESERVATION OF HYDRATED CYSTS OF THE BRINE SHRIMP A. franciscana

被引：0

作者：

Yoshida, Toru ^{[1
]}

Arii, Yasuhiro ^{[1
]}

Hino, Katsuhiko ^{[2
]}

Sawatani, Ikuo ^{[2
]}

Tanaka, Midori ^{[1
]}

Takahashi, Rei ^{[3
]}

Bando, Toru ^{[4
]}

Mukai, Kazuhisa ^{[5
]}

Fukuo, Keisuke ^{[1
]}

机构：

[1] Mukogawa Womens Univ, Dept Nutr & Food Sci, Sch Human Environm Sci, Nishinomiya, Hyogo 6638558, Japan

[2] Hayashibara Biochem Labs Inc, Res Ctr, Glycosci Inst, Naka Ku, Okayama 7028006, Japan

[3] Doshisha Womens Coll Liberal Arts, Dept Clin Pharm, Fac Pharmaceut Sci, Kyoto 6100395, Japan

[4] Kyoto Univ Hosp, Dept Organ Preservat Technol, Sakyo Ku, Kyoto 6068507, Japan

[5] Hayashibara Biochem Labs Inc, Ctr Res & Dev, Kita Ku, Okayama 7000907, Japan

来源：

CRYOLETTERS | 2011年 / 32卷 / 03期

关键词：

cryopreservation; slow freezing; survival rate; trehalose; ARTEMIA-FRANCISCANA; ENCYSTED EMBRYOS; PRESERVATION; TREHALOSE; ANHYDROBIOSIS; WATER; MITOCHONDRIA; DESICCATION; DEPRESSION; PROTEIN;

D O I：

暂无

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cysts of Artemia franciscana are known to be extremely tolerant to UV and ionizing radiation, hypoxia, dryness, osmotic pressure, and temperatures. However, when cysts are hydrated, their resistance to extreme environmental conditions is markedly reduced, and they subsequently enter a developmental sequence. The hatching rate of hydrated cysts declined when they were rapidly frozen after a short period of hydration but slow freezing improved hatching rates after 6-h hydration (1.4 g H2O /g dry wt). We observed that trehalose content in hydrated cysts was greatly reduced up to 6-h time. DSC analysis showed different thermal profiles at two cooling rates, suggesting the formation of a minuscule ice crystal inside the cells. High hatching rates can be obtained from highly hydrated cysts at slow cooling rate.

引用

页码：206 / 215

页数：10

共 32 条

[21]

Paiva C L, 1996, Biotechnol Annu Rev, V2, P293, DOI 10.1016/S1387-2656(08)70015-2

[22] Cryopreservation of mammalian embryos and oocytes: Recent advances [J].