The role of chronic infection in children with otitis media with effusion: Evidence for intracellular persistence of bacteria

被引：53

作者：

Coates, Harvey ^{[1
]}

Thornton, Ruth ^{[1
]}

Langlands, Jennifer ^{[1
]}

Filion, Pierre

Keil, Anthony D.

Vijayasekaran, Shyan

Richmond, Peter ^{[1
]}

机构：

[1] Univ Western Australia, Sch Paediat & Child Hlth, Nedlands, WA 6009, Australia

来源：

OTOLARYNGOLOGY-HEAD AND NECK SURGERY | 2008年 / 138卷 / 06期

关键词：

D O I：

10.1016/j.otohns.2007.02.009

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

OBJECTIVE: Demonstrate mucosal bacterial infection in children with otitis media with effusion (OME). STUDY DESIGN AND SETTING: Middle ear mucosal biopsies from 11 children with OME were examined for bacteria utilizing transmission electron microscopy. This was correlated with standard culture and polymerase chain reaction (PCR) of middle ear effusions. RESULTS: Gram-positive coccal bacteria were demonstrated in middle ear mucosal epithelial cells of 4 of 11 (36%) children. Morphological appearance of bacteria and detection of pneumolysin DNA by PCR in middle ear fluid suggests a role for persistent intracellular infection with Streptococcus pneumoniae and other gram-positive cocci in some cases of OME. CONCLUSION: Intracellular bacterial infection of middle ear mucosal epithelial cells in children with OME may be an important mechanism for bacterial persistence, and contribute to inflammation and mucus production in the pathogenesis of this condition. SIGNIFICANCE: Persistent intracellular infection is a novel paradigm for OME pathogenesis in children and may influence antibiotic effectiveness in treatment of this condition. (C) 2008 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.

引用

页码：778 / 781

页数：4

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