Genome-Wide Identification and Expression Analysis of Dof Transcription Factors in Lotus (Nelumbo nucifera Gaertn.)

被引:6
作者
Cao, Xiaohan [1 ]
Wan, Wenyang [1 ]
Mao, Huimin [1 ]
Yin, Dandan [1 ]
Deng, Xianhui [1 ]
Yan, Huan [1 ]
Ren, Liping [1 ,2 ]
机构
[1] Fuyang Normal Univ, Biol & Food Engn Sch, Key Lab Hort Plant Biol, Fuyang 236037, Peoples R China
[2] Fuyang Acad Agr Sci, Fuyang 236065, Peoples R China
来源
PLANTS-BASEL | 2022年 / 11卷 / 15期
关键词
Nelumbo nucifera; Dof transcription factor; salinity stress; ABIOTIC STRESS RESPONSES; FACTOR FAMILY; DNA-BINDING; FACTOR GENE; SALINITY STRESS; SALT STRESS; GROWTH; ACCUMULATION; PROMOTERS; TOLERANCE;
D O I
10.3390/plants11152057
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Lotus (Nelumbo nucifera Gaertn.) is a traditional Chinese aquatic flower with high ornamental and economic value, but water salinity seriously affects lotus cultivation and distribution. The Dof transcription factors (TFs) play a crucial function in the regulatory network of growth and defense in plants. However, no systematic investigations of the Dof TFs in lotus have been performed. In this study, comprehensive searches of the lotus genome yielded 29 potential NnDofs. We carried out a series of standardized analyses, which include physical properties, multiple sequence alignment, phylogenetic analysis, gene structure, motif composition, cis-acting element prediction, chromosome distribution, and synteny analysis. The results showed that segment duplication probably caused the NnDofs gene family expansion. The potential functions of NnDofs in lotus development and stress conditions are speculated by promoter analysis. Furthermore, a complete expression investigation of NnDofs utilizing an RNA-seq atlas and quantitative real-time polymerase chain reaction (qRT-PCR) was performed. The majority of the NnDofs exhibit tissue-specific expression patterns, and many genes have been identified as being extremely sensitive to salt stressors. Overall, this study is the first to report a genome-wide assessment of the Dof family in lotus, and the findings offer vital insights for prospective functional studies on lotus salinity stress.
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页数:16
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