Promising Multiple-Epitope Recombinant Vaccine against Foot-and-Mouth Disease Virus Type O in Swine

被引：55

作者：

Shao, Jun-Jun ^{[1
]}

Wong, Chung Kai ^{[2
]}

Lin, Tong ^{[1
]}

Lee, Shuk Kwan ^{[2
]}

Cong, Guo-Zheng ^{[1
]}

Sin, Fion Wai Yee ^{[2
]}

Du, Jun-Zheng ^{[1
]}

Gao, Shan-Dian ^{[1
]}

Liu, Xiang-Tao ^{[1
]}

Cai, Xue-Peng ^{[1
]}

Xie, Yong ^{[2
]}

Chang, Hui-Yun ^{[1
]}

Liu, Ji-Xing ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Key Lab Anim Virol, Minist Agr, State Key Lab Vet Etiol Biol,Lanzhou Vet Res Inst, Lanzhou 730046, Peoples R China

[2] Hong Kong Univ Sci & Technol, Dept Biol, Kowloon, Hong Kong, Peoples R China

来源：

CLINICAL AND VACCINE IMMUNOLOGY | 2011年 / 18卷 / 01期

基金：

国家高技术研究发展计划(863计划);

关键词：

T-CELL EPITOPES; PRECURSOR POLYPEPTIDE P1; PARTIAL PROTECTION; SYNTHETIC PEPTIDE; IMMUNE-RESPONSE; NUCLEOTIDE-SEQUENCE; CAPSID PROTEINS; B-CELL; CATTLE; INFECTION;

D O I：

10.1128/CVI.00236-10

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In order to develop a completely safe immunogen to replace the traditional inactivated vaccine, a tandem-repeat multiple-epitope recombinant vaccine against foot-and-mouth disease (FMD) virus (FMDV) type O was developed. It contained three copies each of residues 141 to 160 and 200 to 213 of VP1 of the O/China/99 strain of FMDV coupled with a swine immunoglobulin G heavy-chain constant region (scIgG). The data showed that the multiple-epitope recombinant vaccine elicited high titers of anti-FMDV specific antibodies in swine at 30 days postvaccination (dpv) and conferred complete protection against a challenge with 10(3) 50% swine infective doses of the O/China/99 strain. The anti-FMDV specific antibody titers were not significantly different between the multiple-epitope recombinant vaccine and the traditional vaccine (t test, P > 0.05). The number of 50% pig protective doses was 6.47, which is higher than the number recommended by the World Organization for Animal Health. The multiple-epitope recombinant vaccine resulted in a duration of immunity of at least 6 months. We speculate that the multiple-epitope recombinant vaccine is a promising vaccine that may replace the traditional inactivated vaccine for the prevention and control of FMD in swine in the future.

引用

页码：143 / 149

页数：7

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