Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

被引：26
作者：
Liu, Yixiang [1 ,2 ]
Liu, Wenqiang [1 ]
Wang, Yanbo [3 ]
Ma, Yu [1 ]
Huang, Ling [1 ]
Zou, Chao [1 ]
Li, Donghui [1 ]
Cao, Min-Jie [1 ]
Liu, Guang-Ming [1 ,2 ]
机构：
[1] Jimei Univ, Coll Food & Biol Engn, Xiamen 361021, Peoples R China
[2] Xiamen Key Lab Marine Funct Food, Xiamen 361021, Peoples R China
[3] Zhejiang Gongshang Univ, Sch Food Sci & Biotechnol, Hangzhou 310018, Peoples R China
来源：
MARINE DRUGS | 2019年 / 17卷 / 12期
基金：
国家重点研发计划;
关键词：
sulfated galactans; marine algae; enterotoxigenic Escherichia coli; anti-diarrhea; anti-adhesion; MOLECULAR-WEIGHT FUCOIDAN; ANTIBACTERIAL ACTIVITY; POLYSACCHARIDE; ANTIOXIDANT; MECHANISMS; ADHERENCE; ANTITUMOR; SURFACE; GUM;
D O I：
10.3390/md17120694
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics. This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88. Results showed that the sulfated polysaccharides with molecular weight distribution <= 20.0 kDa exhibited antibacterial activity against ETEC K88. A structure-activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition. In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides. Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion. In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.
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页数：16
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