A CD44-biosensor for evaluating metastatic potential of breast cancer cells based on quartz crystal microbalance

被引：17

作者：

Yang, Xiaojuan ^{[1
]}

Zhou, Rongcheng ^{[1
]}

Hao, Yan ^{[1
]}

Yang, Peihui ^{[1
]}

机构：

[1] Jinan Univ, Dept Chem, Guangzhou 510632, Guangdong, Peoples R China

来源：

SCIENCE BULLETIN | 2017年 / 62卷 / 13期

基金：

中国国家自然科学基金;

关键词：

CD44; Quartz crystal microbalance; Metastatic potential; Breast cancer cells; Stiffness; MECHANICAL CHANGES; QCM BIOSENSOR; CD44; ADHESION; TIME; NANOSTRUCTURES; HYALURONAN; BINDING; AFM;

D O I：

10.1016/j.scib.2017.05.022

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A sensitive CD44-biosensor based on quartz crystal microbalance (QCM) was proposed for evaluating metastatic potential of breast cancer cells by using hyaluronan (HA) functionalized substrate film, polydopamine and polyethyleneimine composite film, for the purpose of capturing CD44-positive cancer cells through specific binding of HA to CD44. Two differently CD44-expressed breast cancer cell lines (MDAMB-231 cells and MCF-7 cells) were put to use as targets for quantitative analysis as well as evaluation of metastatic potential of the cells. The limit of detection for MDA-MB-231 (M231) cells and MCF-7 cells were 300 and 1,000 cells mL(-1), respectively. The expression level of CD44 on M231 cells exhibited two times higher than that of MCF-7 cells, indicating of a higher metastatic potential. Moreover, poly-Llysine modified QCM sensor was applied to monitor the stiffness of breast cancer cells that can reflect metastatic potential of cells. The results revealed that the MCF-7 cells were stiffer than M231 cells, implying that the M231 cells possessed higher metastatic potential. The proposed protocol is simple and rapid to evaluate the metastatic potential of cancer cells, in addition to offering a promising diagnostic tool for metastatic cancer. (C) 2017 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.

引用

页码：923 / 930

页数：8

共 39 条

[1] Single-cell resolution diagnosis of cancer cells by carbon nanotube electrical spectroscopy [J].

Abdolahad, M. ;

Janmaleki, M. ;

Taghinejad, M. ;

Taghnejad, H. ;

Salehi, F. ;

Mohajerzadeh, S. .

NANOSCALE, 2013, 5 (08) :3421-3427

[2] Electrochemical Switching with 3D DNA Tetrahedral Nanostructures Self-Assembled at Gold Electrodes [J].

Abi, Alireza ;

Lin, Meihua ;

Pei, Hao ;

Fan, Chunhai ;

Ferapontova, Elena E. ;

Zuo, Xiaolei .

ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (11) :8928-8931

[3] Quartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptors [J].

Atay, Seda ;

Piskin, Kevser ;

Yilmaz, Fatma ;

Cakir, Canan ;

Yavuz, Handan ;

Denizli, Adil .

ANALYTICAL METHODS, 2016, 8 (01) :153-161

[4] DDB2 (damaged-DNA binding 2) protein: a new modulator of nanomechanical properties and cell adhesion of breast cancer cells [J].

Barbieux, Claire ;

Bacharouche, Jalal ;

Soussen, Charles ;

Hupont, Sebastien ;

Razafitianamaharavo, Angelina ;

Klotz, Remi ;

Pannequin, Remi ;

Brie, David ;

Becuwe, Philippe ;

Francius, Gregory ;

Grandemange, Stephanie .

NANOSCALE, 2016, 8 (09) :5268-5279

[5] CD44 as a potential diagnostic tumor marker [J].

Basakran, Nawwaf S. .

SAUDI MEDICAL JOURNAL, 2015, 36 (03) :273-279

[6] Detection of apoptosis and drug resistance of human breast cancer cells to taxane treatments using quartz crystal microbalance biosensor technology [J].

Braunhut, SJ ;

McIntosh, D ;

Vorotnikova, E ;

Zhou, T ;

Marx, KA .

ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2005, 3 (01) :77-88

[7] CD44 enhances the epithelial-mesenchymal transition in association with colon cancer invasion [J].

Cho, Sang Hyuk ;

Park, Yeon Sun ;

Kim, Hun Jin ;

Kim, Chang Hyun ;

Lim, Sang Woo ;

Huh, Jung Wook ;

Lee, Jae Hyuk ;

Kim, Hyeong Rok .

INTERNATIONAL JOURNAL OF ONCOLOGY, 2012, 41 (01) :211-218

[8] BINDING AND DEGRADATION OF HYALURONAN BY HUMAN BREAST-CANCER CELL-LINES EXPRESSING DIFFERENT FORMS OF CD44 - CORRELATION WITH INVASIVE POTENTIAL [J].

CULTY, M ;

SHIZARI, M ;

THOMPSON, EW ;

UNDERHILL, CB .

JOURNAL OF CELLULAR PHYSIOLOGY, 1994, 160 (02) :275-286

[9] The tumor cell-host organ interface in the early onset of metastatic organ colonisation [J].

Gassmann, Peter ;

Haier, Joerg .

CLINICAL & EXPERIMENTAL METASTASIS, 2008, 25 (02) :171-181

[10] Deformability study of breast cancer cells using microfluidics [J].

Hou, H. W. ;

Li, Q. S. ;

Lee, G. Y. H. ;

Kumar, A. P. ;

Ong, C. N. ;

Lim, C. T. .

BIOMEDICAL MICRODEVICES, 2009, 11 (03) :557-564

← 1 2 3 4 →