Intuitive Label-Free SERS Detection of Bacteria Using Aptamer-Based in Situ Silver Nanoparticles Synthesis

被引：143

作者：

Gao, Weicun ^{[1
,2
]}

Li, Bo ^{[1
]}

Yao, Ruizhi ^{[3
]}

Li, Zhiping ^{[1
]}

Wang, Xiwen ^{[1
]}

Dong, Xiaolin ^{[1
]}

Qu, Han ^{[1
]}

Li, Qianxue ^{[1
]}

Li, Nan ^{[1
]}

Chi, Hang ^{[1
]}

Zhou, Bo ^{[1
]}

Xia, Zhiping ^{[1
]}

机构：

[1] Acad Mil Med Sci, Key Lab Jilin Prov Zoonosis Prevent & Control, Inst Mil Vet, Changchun 130122, Jilin, Peoples R China

[2] Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Jilin, Peoples R China

[3] Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 163000, Heilongjiang, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2017年 / 89卷 / 18期

关键词：

ENHANCED RAMAN-SCATTERING; STAPHYLOCOCCUS-AUREUS; DNA APTAMER; GOLD NANOPARTICLES; SPECTROSCOPY; IDENTIFICATION; CELLS; DISCRIMINATION; SELECTION; THROMBIN;

D O I：

10.1021/acs.analchem.7b01813

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The characteristic of an ideal bacteria-detection method should have high sensitivity and specificity, be easy to operate, and not have a time-consuming culture process. In this study, we report a new bacteria-detection strategy that can recognize bacteria quickly and directly by surface-enhanced Raman scattering (SERS) with the formation of well-defined bacteria-aptarner@AgNPs. SERS signals generated by bacteria-aptamer@AgNPs exhibited a linear dependence on bacteria (R-2 = 0.9671) concentration ranging from 10(1) to 10(7) cfu/mL. The detection limit is sensitive down to 1.5 cfu/mL. Meanwhile, the bacteria SERS signal was dramatically enhanced by its specifically recognized aptamer, and the bacteria could be identified directly and visually through the SERS spectrum. This strategy eliminates the puzzling data analysis of previous studies and offers significant advantages over existing approaches, getting a critical step toward the creation of SERS-based biochips for rapid in situ bacteria detection in mixture samples.

引用

页码：9836 / 9842

页数：7

共 38 条

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