Analytical approaches to investigate transmitter content and release from single secretory vesicles

被引：22
作者：
Omiatek, Donna M. [2 ]
Cans, Ann-Sofie [3 ]
Heien, Michael L. [2 ]
Ewing, Andrew G. [1 ,2 ]
机构：
[1] Univ Gothenburg, Dept Chem, S-41296 Gothenburg, Sweden
[2] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
[3] Chalmers, Dept Biol & Chem Engn, S-41296 Gothenburg, Sweden
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2010年 / 397卷 / 08期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
Vesicle; Exocytosis; Neurotransmitter; Carbon-fiber amperometry; Electrochemical cytometry; Capillary electrophoresis; INTERNAL-REFLECTION FLUORESCENCE; FROG NEUROMUSCULAR-JUNCTION; PHEOCHROMOCYTOMA PC12 CELLS; ADRENAL CHROMAFFIN CELLS; QUANTAL SIZE; FUSION PORE; CAPILLARY-ELECTROPHORESIS; SYNAPTIC-TRANSMISSION; EXOCYTOTIC RELEASE; PATCH AMPEROMETRY;
D O I：
10.1007/s00216-010-3698-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The vesicle serves as the primary intracellular unit for the highly efficient storage and release of chemical messengers triggered during signaling processes in the nervous system. This review highlights conventional and emerging analytical methods that have used microscopy, electrochemistry, and spectroscopy to resolve the location, time course, and quantal content characteristics of neurotransmitter release. Particular focus is on the investigation of the synaptic vesicle and its involvement in the fundamental molecular mechanisms of cell communication.
引用
收藏
页码：3269 / 3279
页数：11
相关论文
共 74 条
  • [1] The exocytotic event in chromaffin cells revealed by patch amperometry
    Albillos, A
    Dernick, G
    Horstmann, H
    Almers, W
    deToledo, GA
    Lindau, M
    [J]. NATURE, 1997, 389 (6650) : 509 - 512
  • [2] Relationship between amperometric pre-spike feet and secretion granule composition in Chromaffin cells: An overview
    Amatore, Christian
    Arbault, Stphane
    Bonifas, Imelda
    Guille, Manon
    Lemaitre, Fredric
    Verchier, Yann
    [J]. BIOPHYSICAL CHEMISTRY, 2007, 129 (2-3) : 181 - 189
  • [3] Comparison of apex and bottom secretion efficiency at chromaffin cells as measured by amperometry
    Amatore, Christian
    Arbault, Stephane
    Lemaitre, Frederic
    Verchier, Yann
    [J]. BIOPHYSICAL CHEMISTRY, 2007, 127 (03) : 165 - 171
  • [4] Calculation of transmitter concentration in individual PC12 cell vesicles with electrochemical data and a distribution of vesicle size obtained by electron microscopy
    Anderson, BB
    Zerby, SE
    Ewing, AG
    [J]. JOURNAL OF NEUROSCIENCE METHODS, 1999, 88 (02) : 163 - 170
  • [5] Single synaptic vesicles fusing transiently and successively without loss of identity
    Aravanis, AM
    Pyle, JL
    Tsien, RW
    [J]. NATURE, 2003, 423 (6940) : 643 - 647
  • [6] CELL-SUBSTRATE CONTACTS ILLUMINATED BY TOTAL INTERNAL-REFLECTION FLUORESCENCE
    AXELROD, D
    [J]. JOURNAL OF CELL BIOLOGY, 1981, 89 (01) : 141 - 145
  • [7] Quantitative measurement of serotonin synthesis and sequestration in individual live neuronal cells
    Balaji, J
    Desai, R
    Kaushalya, SK
    Eaton, MJ
    Maiti, S
    [J]. JOURNAL OF NEUROCHEMISTRY, 2005, 95 (05) : 1217 - 1226
  • [8] OPTICAL ANALYSIS OF SYNAPTIC VESICLE RECYCLING AT THE FROG NEUROMUSCULAR-JUNCTION
    BETZ, WJ
    BEWICK, GS
    [J]. SCIENCE, 1992, 255 (5041) : 200 - 203
  • [9] ACTIVITY-DEPENDENT FLUORESCENT STAINING AND DESTAINING OF LIVING VERTEBRATE MOTOR-NERVE TERMINALS
    BETZ, WJ
    MAO, F
    BEWICK, GS
    [J]. JOURNAL OF NEUROSCIENCE, 1992, 12 (02) : 363 - 375
  • [10] Pharmacological regulation of the late steps of exocytosis
    Borges, R
    Machado, JD
    Betancor, G
    Camacho, M
    [J]. CHROMAFFIN CELL: TRANSMITTER BIOSYNTHESIS, STORAGE, RELEASE, ACTIONS, AND INFORMATICS, 2002, 971 : 184 - 192
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