Expression of growth control and differentiation genes in human lens epithelial cells with extended life span

被引:0
|
作者
Fleming, TP
Song, Z
Andley, UP
机构
[1] Washington Univ, Sch Med, Dept Ophthalmol & Visual Sci, St Louis, MO 63110 USA
[2] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA
[3] Washington Univ, Sch Med, Dept Biochem & Mol Biophys, St Louis, MO 63110 USA
关键词
D O I
暂无
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PURPOSE. Peptide growth factors including hepatocyte growth factor (HGF), platelet-derived growth factor (PDGF), and epidermal growth factor (EGF) are mitogens for many cell types and may act as regulators of lens epithelial cell growth and differentiation. The present study was undertaken to investigate the expression of growth factor receptors and crystallin genes in the human lens epithelial cell line HLE B-3, created by infection with Adeno12-simian virus 40 (Ad12-SV40) hybrid virus. METHODS. Reverse transcriptase-polymerase chain reaction (RT-PCR) with gene-specific primers was used to detect transcripts, and Southern and western blot analyses were used for identification of gene products. Functional analysis of PDGF receptor was performed by measuring the effect of PDGF-BB on Ca2+ release, cell growth, and western blot analysis, by using an antiphosphotyrosine antibody. RESULTS. Human lens epithelial B-3 cells expressed the growth factor receptors HGF-R, EGF-R, and PDGF-R beta, but not PDGF-R alpha, and also expressed the oncogenes H-ras and raf and the growth inhibitor transforming growth factor-beta(1). stimulation of PDGF-R beta with PDGF-BB in HLE B-3 cells increased phosphorylation of the receptor, was associated with an increase in intracellular Ca2+ levels, and produced a small increase in cell growth. In addition, HLE B-3 cells expressed transcripts for alpha A-, alpha B, and beta B2-crystallins, and expressed the corresponding proteins. The transcripts for alpha A-crystallin decreased markedly at higher passages. CONCLUSIONS. The above findings suggest that the increased growth potential of human lens epithelial cells by Ad12-SV40 infection maintained certain lens-specific properties and response to PDGF.
引用
收藏
页码:1387 / 1398
页数:12
相关论文
共 50 条
  • [31] C-met expression and hepatocyte growth factor function in human lens epithelial cells
    Wormstone, IM
    Tamiya, S
    Marcantonio, JM
    Warn, RM
    Duncan, G
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1999, 40 (04) : S562 - S562
  • [32] Ideal lipid profile and genes for an extended life span
    Kolovou, Genovefa
    Kolovou, Vana
    Vasiliadis, Ioannis
    Wierzbicki, Anthony S.
    Mikhailidis, Dimitri P.
    CURRENT OPINION IN CARDIOLOGY, 2011, 26 (04) : 348 - 355
  • [33] GROWTH-CONTROL OF CORNEA AND LENS EPITHELIAL-CELLS
    REID, TW
    PROGRESS IN RETINAL AND EYE RESEARCH, 1994, 13 (02) : 507 - 554
  • [34] Effect of oxLDL on transcriptional expression of human lens epithelial cells
    Zhang, Ru
    Fang, Yang
    Bai, Suwen
    Gao, Huiwen
    Chen, Hongbo
    Zhang, Zhiguo
    Du, Juan
    Shen, Bing
    Wang, Yong
    INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY, 2020, 13 (04): : 655 - 663
  • [35] In vitro growth of human lens epithelial cells in chamber slides
    Schmidt, JF
    Meyer, JH
    Flugel, B
    Hansen, LL
    Loffler, KU
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1996, 37 (03) : 4521 - 4521
  • [36] Effect of growth factors and cytokines on human lens epithelial cells
    Yuge, K
    Okamoto, H
    Ibaraki, N
    Oharazawa, H
    Lin, LR
    Shinohara, T
    Reddy, VN
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1997, 38 (04) : 200 - 200
  • [37] STUDY OF HUMAN SENILE CATARACT - GROWTH AND DIFFERENTIATION OF LENS EPITHELIAL-CELLS IN INVITRO-CELL CULTURE
    EGUCHI, G
    KODAMA, R
    OPHTHALMIC RESEARCH, 1979, 11 (5-6) : 308 - 315
  • [38] CHANGES OF FIBER DIFFERENTIATION IN HUMAN LENS EPITHELIAL-CELLS EXPOSED TO GROWTH-FACTORS IN SEVERAL SUBCULTURES
    REDDY, VN
    IBARAKI, N
    LIN, LR
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 1995, 36 (04) : S260 - S260
  • [39] Expression of matrix metalloproteinases of human lens epithelial cells in the cultured lens capsule bag
    Li, J. H.
    Wang, N. L.
    Wang, J. J.
    EYE, 2008, 22 (03) : 439 - 444
  • [40] Expression of matrix metalloproteinases of human lens epithelial cells in the cultured lens capsule bag
    J H Li
    N L Wang
    J J Wang
    Eye, 2008, 22 : 439 - 444