Ubiquinol promotes retinal ganglion cell survival and blocks the apoptotic pathway in ischemic retinal degeneration

被引：24

作者：

Ju, Won-Kyu ^{[1
]}

Shim, Myoung Sup ^{[1
,3
]}

Kim, Keun-Young ^{[2
]}

Bu, Jung Hyun ^{[1
]}

Park, Tae Lim ^{[1
]}

Ahn, Sangphil ^{[1
]}

Weinreb, Robert N. ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Ophthalmol, Hamilton Glaucoma Ctr, Shiley Eye Inst, 9415 Campus Point Dr, La Jolla, CA 92037 USA

[2] Univ Calif San Diego, Dept Neurosci, Natl Ctr Microscopy & Imaging Res, La Jolla, CA 92093 USA

[3] Duke Univ, Dept Ophthalmol, Durham, NC USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 503卷 / 04期

关键词：

Ubiquinol; Retina; lschemia; Retinal ganglion cell; Apoptosis; TRIGGERS OPA1 RELEASE; COENZYME Q(10); OXIDATIVE STRESS; NEURODEGENERATIVE DISEASES; MITOCHONDRIAL ALTERATION; TRANSIENT ISCHEMIA; PROAPOPTOTIC BAX; OPTIC-NERVE; IN-VIVO; DEATH;

D O I：

10.1016/j.bbrc.2018.08.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coenzyme Q10 (CoQ(10)) protects retinal ganglion cells (RGCs) in experimental retinal ischemia and glaucoma by scavenging reactive oxygen species. We tested whether a diet supplemented with ubiquinol, the reduced form of CoQ(10), promotes RGC survival and blocks the apoptotic pathway in ischemic mouse retina induced by acute high intraocular pressure (IOP) elevation. Ubiquinol (1%) treatment significantly promoted RGC survival at 2 weeks after ischemia/reperfusion. The ubiquinol treatment significantly blocked activation of astroglial and microglial cells in the ischemic retina at 2 weeks. While the ubiquinol treatment significantly decreased active Bax protein expression in the ischemic retina, phosphorylation of Bad at serine 112 and Bcl-xL protein expression were preserved in the ubiquinol-treated ischemic retina at 12 h. Consistently, the ubiquinol treatment prevented apoptotic cell death by blocking caspase-3 cleavage. These results suggest that the ubiquinol enhances RGC survival by modulating the Bax/Bad/Bcl-xL-mediated apoptotic pathway in the ischemic retina. Ubiquinol has therapeutic potential for ameliorating elevated IOP-induced ischemic retinal degeneration. (C) 2018 Elsevier Inc. All rights reserved.

引用

页码：2639 / 2645

页数：7

共 31 条

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