Amphoteric hydrogels for immobilization of enzymes using template polymerization technique

Novel ionizable amphoteric hydrogels were prepared from poly(acrylic acid) and dimethylaminoethyl methacrylate monomer, employing template polymerization technique. The mode of interaction, as proved by FTIR, was multiple H-bonding between the tertiary amino group of the monomer and the carboxylic groups of the polymer. The impact of varying equal polymer-monomer feed ratios from 0.1 to 1.1 on the swelling dynamics was examined. Penetrant sorption experiments demonstrated that the swelling behavior depends strongly on the polymer complex composition. The polymer complex of feed ratio 0.5 : 0.5 (polymer : monomer) showed maximum swelling percentage. The mechanism of the polymer complexes swelling was probably a non-Fickian with n values approaching Fickian behavior. The hydrogels showed maximum swelling efficiencies of 27 folds and 13.5 folds in drastic acidic and basic medium, respectively, using polymer complex of 0.5 : 0.5 feed ratio. Because of reversibility and rapidity of swelling, the gel could be considered as a mechanochemical system. The prepared hydrogel successfully immobilized the industrially used Pgalactosidase as an acidic model enzyme. The novel immobilized enzyme showed a remarkable improvement in its activity (13.8 mu mol min(-1) mg(-1)) compared to the free enzyme (3.2 mu mol min(-1) mg(-1)). The optimum pH values for free and immobilized enzyme were 4.5-5 and 4, respectively.