Decreased osteogenesis of adult mesenchymal stem cells by reactive oxygen species under cyclic stretch: a possible mechanism of age related osteoporosis

被引：89
作者：
Tan, Jiali [1 ,2 ]
Xu, Xin [3 ]
Tong, Zhongchun [1 ,2 ]
Lin, Jiong [1 ,2 ]
Yu, Qiujun [4 ]
Lin, Yao [1 ,2 ]
Kuang, Wei [5 ]
机构：
[1] Sun Yat Sen Univ, Hosp Stomatol, Guanghua Sch Stomatol, Dept Orthodont, Guangzhou 510275, Guangdong, Peoples R China
[2] Guangdong Prov Key Lab Stomatol, Guangzhou, Guangdong, Peoples R China
[3] Sichuan Univ, West China Hosp Stomatol, State Key Lab Oral Dis, Chengdu 610064, Peoples R China
[4] Harvard Univ, Brigham & Womens Hosp, Sch Med, Dept Med,Div Cardiovasc Med, Boston, MA 02115 USA
[5] Guangzhou Gen Hosp, Guangzhou Mil Command, Dept Stomatol, Guangzhou, Guangdong, Peoples R China
来源：
BONE RESEARCH | 2015年 / 3卷
基金：
中国国家自然科学基金;
关键词：
STRAIN; DIFFERENTIATION; MARROW; ADIPOGENESIS; EXPRESSION; THERAPIES; GROWTH;
D O I：
10.1038/boneres.2015.3
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Age related defect of the osteogenic differentiation of mesenchymal stem cells (MSCs) plays a key role in osteoporosis. Mechanical loading is one of the most important physical stimuli for osteoblast differentiation. Here, we compared the osteogenic potential of MSCs from young and adult rats under three rounds of 2 h of cyclic stretch of 2.5% elongation at 1 Hz on 3 consecutive days. Cyclic stretch induced a significant osteogenic differentiation of MSCs from young rats, while a compromised osteogenesis in MSCs from the adult rats. Accordingly, there were much more reactive oxygen species (ROS) production in adult MSCs under cyclic stretch compared to young MSCs. Moreover, ROS scavenger N-acetylcysteine rescued the osteogenic differentiation of adult MSCs under cyclic stretch. Gene expression analysis revealed that superoxide dismutase 1 (SOD1) was significantly downregulated in those MSCs from adult rats. In summary, our data suggest that reduced SOD1 may result in excessive ROS production in adult MSCs under cyclic stretch, and thus manipulation of the MSCs from the adult donors with antioxidant would improve their osteogenic ability.
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页数：6
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