Sublingual immunization induces broad-based systemic and mucosal immune responses in mice

被引：166

作者：

Cuburu, Nicolas ^{[2
]}

Kweon, Mi-Na ^{[1
]}

Song, Joo-Hye ^{[1
]}

Hervouet, Catherine ^{[2
]}

Luci, Carmelo ^{[2
]}

Sun, Jia-Bin ^{[3
,4
]}

Hofman, Paul ^{[5
]}

Holmgren, Jan ^{[3
,4
]}

Anjuere, Fabienne ^{[2
]}

Czerkinsky, Cecil ^{[1
,2
]}

机构：

[1] Int Vaccine Inst, Div Sci Lab, Seoul 151919, South Korea

[2] Univ Nice Sophia Antipolis, INSERM, UMR 721, Nice, France

[3] Vaccine Res Inst, Dept Microbiol & Immunol, Gothenburg, Sweden

[4] Univ Gothenburg, Vaccine Res Inst, Gothenburg, Sweden

[5] INSERM ERI 21, Nice, France

来源：

VACCINE | 2007年 / 25卷 / 51期

关键词：

sublingual mucosa; cholera toxin; mucosal vaccine; antibody-secreting cells; cytotoxic T lymphocytes; influenza;

D O I：

10.1016/j.vaccine.2007.09.073

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The potential of sublingual (s.l.) delivery of vaccine was examined in mice. We show the existence of a dense network of dendritic cells (DCs) in the s.l. epithelium and a rapid and transient increase in the frequency of s.l. DCs after topical application of cholera toxin (CT) adjuvant under the tongue. S.l. immunization with ovalbumin and CT induced vigorous systemic and mucosal antibody responses. Such treatment promoted mixed Th1 and Th2 cytokine responses and induced cytotoxic CD8(+) T cells in lung tissues and in systemic lymphoid organs. S.l. immunization was comparable to intranasal immunization and was superior to oral immunization regarding the magnitude and anatomic dissemination of the induced immune responses. S.l. administration of live influenza virus at a dose lethal by the nasal route was well tolerated and did not redirect virus to the olfactory bulb. These features underscore the potential of the s.l. mucosa to serve as an alternative vaccine delivery route. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：8598 / 8610

页数：13

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