Enzyme Biosensors for Biomedical Applications: Strategies for Safeguarding Analytical Performances in Biological Fluids

被引：332

作者：

Rocchitta, Gaia ^{[1
]}

Spanu, Angela ^{[1
]}

Babudieri, Sergio ^{[1
]}

Latte, Gavinella ^{[1
]}

Madeddu, Giordano ^{[1
]}

Galleri, Grazia ^{[1
]}

Nuvoli, Susanna ^{[1
]}

Bagella, Paola ^{[1
]}

Demartis, Maria Ilaria ^{[1
]}

Fiore, Vito ^{[1
]}

Manetti, Roberto ^{[1
]}

Serra, Pier Andrea ^{[1
]}

机构：

[1] Univ Sassari, Sch Med, Dept Clin & Expt Med, Viale S Pietro 43-B, I-07100 Sassari, Italy

来源：

SENSORS | 2016年 / 16卷 / 06期

关键词：

biosensor; biomedical applications; amperometry; biological fluids; interferents; REDUCED GRAPHENE OXIDE; AMPEROMETRIC BIOSENSOR; SOL-GEL; GOLD NANOPARTICLES; L-LACTATE; IMMOBILIZATION STRATEGIES; GLUCOSE-DEHYDROGENASE; ALCOHOL-DEHYDROGENASE; CARBON NANOTUBES; IN-VIVO;

D O I：

10.3390/s16060780

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Enzyme-based chemical biosensors are based on biological recognition. In order to operate, the enzymes must be available to catalyze a specific biochemical reaction and be stable under the normal operating conditions of the biosensor. Design of biosensors is based on knowledge about the target analyte, as well as the complexity of the matrix in which the analyte has to be quantified. This article reviews the problems resulting from the interaction of enzyme-based amperometric biosensors with complex biological matrices containing the target analyte(s). One of the most challenging disadvantages of amperometric enzyme-based biosensor detection is signal reduction from fouling agents and interference from chemicals present in the sample matrix. This article, therefore, investigates the principles of functioning of enzymatic biosensors, their analytical performance over time and the strategies used to optimize their performance. Moreover, the composition of biological fluids as a function of their interaction with biosensing will be presented.

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