Evaluation of environmental impact on natural populations of the Mediterranean killifish Aphanius fasciatus by quantitative RNA biomarkers

被引：30

作者：

Kessabi, Kaouthar ^{[2
]}

Navarro, Anna ^{[1
]}

Casado, Marta ^{[1
]}

Saied, Khaled ^{[2
]}

Messaoudi, Imed ^{[2
]}

Pina, Benjamin ^{[1
]}

机构：

[1] CSIC, IDAEA, Inst Environm Assessment & Water Res, ES-08034 Barcelona, Spain

[2] Univ Monastir, Inst Biotechnol, Monastir 5000, Tunisia

来源：

MARINE ENVIRONMENTAL RESEARCH | 2010年 / 70卷 / 3-4期

关键词：

Cyprinodontiformes; Brackish waters; Quantitative real-time polymerase chain reaction (qRT-PCR); Pollution markers; mRNA quantitation; Gene expression; Western Mediterranean Sea; Sentinel species; METALLOTHIONEIN MESSENGER-RNA; ZEBRAFISH DANIO-RERIO; DIFFERENTIAL GENE-EXPRESSION; O-DEETHYLASE EROD; REAL-TIME PCR; SPINAL DEFORMITIES; OXIDATIVE STRESS; ATLANTIC SALMON; RT-PCR; MORPHOLOGICAL DEFORMITIES;

D O I：

10.1016/j.marenvres.2010.06.005

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The Mediterranean killifish, Aphanius fasciatus (Valenciennes, 1821) represents an excellent sentinel species for analysis of environmental impact in coastal areas. Quantitative changes on liver mRNA levels of five stress-related genes - metallothionein (metal exposure), vitellogenin, (estrogenic effects), cytochrome P4501A (CYP1A, dioxin-like compounds), superoxide dismutase (oxidative stress) and HSP70 (general stress) - were tested as markers of exposure to pollutants both in natural populations and in experimental treatments with model effectors. Analysis of fish from a metal-contaminated site in the Tunisian coast (Sfax) indicated that fish presenting spinal deformities (eight times more frequent at this site than in reference sites) showed increased levels of hepatic CYP1A mRNA levels compared to non-deformed fish from the same site or from the reference Louza site, suggesting a combined impact by metals and organic pollutants. Due to the strategy used in their design, these quantitative RNA markers will likely be useful to analyze environmental impacts on other related fish species. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：327 / 333

页数：7

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