Agromyces insulae sp nov., an actinobacterium isolated from a soil sample

A novel Gram-reaction-positive, non-motile, aerobic bacterium, designated CFH S0483(T), was isolated from a soil sample collected from Catba island in Halong Bay, Vietnam. 16S rRNA gene sequence analysis showed that the strain is a member of the genus Agromyces and has highest 16S rRNA gene sequence similarities with Agromyces humatus DSM 16389(T) (97.3 %) and Agromyces ramosus DSM 43045(T) (97.1 %), and similarities <97.0 % with type strains of other species of the genus Agromyces. Strain CFH S0483(T) was able to grow at 10-37 degrees C, at pH 7.0-9.0 and tolerated NaCl up to 2.0 % (w/v). The whole-cell sugars were mannose, galactose, glucose and ribose. The isolate contained L-2,4-diaminobutyric acid, D-alanine, D-glutamic acid and glycine in the cell-wall peptidoglycan. Strain CFH S0483(T) exhibited a menaquinone system with MK-12, and the major fatty acids were anteiso-C-15 : 0, anteiso-C-17 : 0 and iso-C-16 : 0. The genomic DNA G+C content of strain CFH S0483(T) was 71.6 mol%. Based on the phylogenetic and phenotypic analysis, and low DNA-DNA hybridization values, strain CFH S0483(T) could not be classified into any recognized species of the genus Agromyces. Strain CFH S0483(T) is therefore considered to represent a novel species of the genus Agromyces, for which the name Agromyces insulae sp. nov. is proposed. The type strain is CFH S0483(T) (=KCTC 39117(T) =CCTCC AB 2014301(T)).