CD4+ CD28null cells are expanded and exhibit a cytolytic profile in end-stage renal disease patients on peritoneal dialysis

被引:30
作者
Yadav, Ashok Kumar [1 ]
Jha, Vivekanand [1 ]
机构
[1] Postgrad Inst Med Educ & Res, Dept Nephrol, Chandigarh 160012, India
关键词
cardiovascular disease; end-stage renal disease; inflammation; peritoneal dialysis; T cells; CD4(+)CD28(NULL) T-CELLS; ACUTE CORONARY SYNDROMES; RHEUMATOID-ARTHRITIS PATIENTS; CARDIOVASCULAR-DISEASE; PERSISTENT INFECTION; UNSTABLE ANGINA; INFLAMMATION; LYMPHOCYTES; EXPRESSION; REPERTOIRE;
D O I
10.1093/ndt/gfr010
中图分类号
R3 [基础医学]; R4 [临床医学];
学科分类号
1001 ; 1002 ; 100602 ;
摘要
Background. End-stage renal failure patients, including those on peritoneal dialysis (PD), exhibit several nontraditional cardiovascular (CV) risk factors. A role of CD4(+)CD28(null) T lymphocytes in the genesis of coronary artery disease (CAD) has been proposed. We investigated this cell population and examined its phenotype in a cohort of PD patients without CV disease. Methods. The frequency of the peripheral blood CD4+ CD28null T-cell compartment and cytotoxic characteristic of these cells (as assessed by perforin and granzyme B expressions) were determined in 33 PD patients without CAD and 20 healthy subjects by two-and three-color flow-cytometry. High-sensitivity C-reactive protein (hs-CRP) was determined by enzyme-linked immunosorbent assay. We investigated whether there was a correlation between these cells and traditional CV risk factors. Results. Compared to healthy controls, CD4(+)CD28(null) T cells were significantly expanded in PD patients (10.30 +/- 2.03% versus 3.55 +/- 0.67%, mean +/- SE, P = 0.0007). Perforin and granzyme B expressions were restricted to CD4(+)CD28(null) T cells compared to CD4(+)CD28(+) T cells (< 0.0001). A greater proportion of CD4(+)CD28(null) T cells in PD patients expressed these molecules (P = 0.007 and 0.04). hs-CRP level was increased in PD patients (P < 0.0001) but did not correlate with the CD4(+)CD28(null) T-cell frequency. Increasing age correlated with the CD4(+)CD28(null) cells. Conclusions. PD patients exhibit a substantially increased number of circulating CD4(+)CD28(null) T cells that show a cytolytic profile before the onset of clinically significant CAD. Their significance as a nontraditional CV risk factor needs further studies.
引用
收藏
页码:1689 / 1694
页数:7
相关论文
共 28 条
[1]   Neopterin, CD4+CD28-lymphocytes and the extent and severity of coronary artery disease [J].
Alber, Hannes F. ;
Duftner, Christina ;
Wanitschek, Maria ;
Doerler, Jakob ;
Schirmer, Michael ;
Suessenbacher, Alois ;
Frick, Matthias ;
Dichtl, Wolfgang ;
Pachinger, Otmar ;
Weidinger, Franz .
INTERNATIONAL JOURNAL OF CARDIOLOGY, 2009, 135 (01) :27-35
[2]   CD8dim and NKG2D Expression Defines Related Subsets of CD4+ T cells in HIV-Infected Patients With Worse Prognostic Factors [J].
Alonso-Arias, Rebeca ;
Lopez-Vazquez, Antonio ;
Diaz-Pena, Roberto ;
Sampere, Angeles ;
Tricas, Lourdes ;
Asensi, Victor ;
Rodrigo, Luis ;
Lopez-Larrea, Carlos .
JAIDS-JOURNAL OF ACQUIRED IMMUNE DEFICIENCY SYNDROMES, 2009, 51 (04) :390-398
[3]   Characterization of the CD4+T cell response Epstein-Barr virus during primary and persistent infection [J].
Amyes, E ;
Hatton, C ;
Montamat-Sicotte, D ;
Gudgeon, N ;
Rickinson, AB ;
McMichael, AJ ;
Callan, MFC .
JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 198 (06) :903-911
[4]   Expansion of cytolytic CD4+CD28- T cells in end-stage renal disease [J].
Betjes, Michiel G. H. ;
Huisman, Martin ;
Weimar, Willem ;
Litjens, Nicolle H. R. .
KIDNEY INTERNATIONAL, 2008, 74 (06) :760-767
[5]   Circulating pro-inflammatory CD4posCD28null T cells are independently associated with cardiovascular disease in ESRD patients [J].
Betjes, Michiel G. H. ;
de Wit, Elly E. A. ;
Weimar, Willem ;
Litjens, Nicolle H. R. .
NEPHROLOGY DIALYSIS TRANSPLANTATION, 2010, 25 (11) :3640-3646
[6]   CMV Seropositivity Determines Epoetin Dose and Hemoglobin Levels in Patients with CKD [J].
Betjes, Michiel G. H. ;
Weimar, Willem ;
Litjens, Nicolle H. R. .
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 2009, 20 (12) :2661-2666
[7]  
Chiu Y W, 2010, Indian J Nephrol, V20, P59, DOI 10.4103/0971-4065.65296
[8]   Skewed distribution of proinflammatory CD4+CD28null T cells in rheumatoid arthritis [J].
Fasth, Andreas Er ;
Snir, Omri ;
Johansson, Anna At ;
Nordmark, Birgitta ;
Rahbar, Afsar ;
af Klint, Erik ;
Bjorkstrom, Niklas K. ;
Ulfgren, Ann-Kristin ;
van Vollenhoven, Ronald F. ;
Malmstrom, Vivianne ;
Trollmo, Christina .
ARTHRITIS RESEARCH & THERAPY, 2007, 9 (05)
[9]   CD4+CD28-T lymphocytes contribute to early atherosclerotic damage in rheumatoid arthritis patients [J].
Gerli, R ;
Schillaci, G ;
Giordano, A ;
Bocci, EB ;
Bistoni, O ;
Vaudo, G ;
Marchesi, S ;
Pirro, M ;
Ragni, F ;
Shoenfeld, Y ;
Mannarino, E .
CIRCULATION, 2004, 109 (22) :2744-2748
[10]   Mechanisms of disease - Inflammation, atherosclerosis, and coronary artery disease [J].
Hansson, GK .
NEW ENGLAND JOURNAL OF MEDICINE, 2005, 352 (16) :1685-1695