Quantum-Dot-Based FRET Detection of Histone Acetyltransferase Activity

被引：58
作者：
Ghadiali, James E.
Lowe, Stuart B.
Stevens, Molly M. [1 ]
机构：
[1] Univ London Imperial Coll Sci Technol & Med, Dept Mat, Dept Bioengn, London SW7 2AZ, England
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2011年 / 50卷 / 15期
基金：
英国工程与自然科学研究理事会;
关键词：
biosensors; enzymes; epigenetics; FRET; nanoparticles; RESONANCE ENERGY-TRANSFER; MODIFYING ENZYMES; ANACARDIC ACID; ASSAY; EPIGENETICS; INHIBITORS; DISEASE; KINASE; P300; ASSEMBLIES;
D O I：
10.1002/anie.201008263
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Straightforward and label-free: A simple method for the detection of histone-modifying enzymes is based on quantum dot (QD) Förster resonance energy transfer (FRET) donors. Histone acetyltransferase activity can be measured via the enzyme-dependent assembly of a quantum dot/peptide immunocomplex (see picture; His=histidine), which can be conducted in a homogeneous reaction without the need for complicated surface derivatization procedures. Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：3417 / 3420
页数：4
相关论文
共 38 条
[1]   Assays for the identification and evaluation of histone acetyltransferase inhibitors [J].
Aherne, GW ;
Rowlands, MG ;
Stimson, L ;
Workman, P .
METHODS, 2002, 26 (03) :245-253
[2]   Hybrid Nanoparticle-Liposome Detection of Phospholipase Activity [J].
Aili, Daniel ;
Mager, Morgan ;
Roche, David ;
Stevens, Molly M. .
NANO LETTERS, 2011, 11 (04) :1401-1405
[3]   Small molecule modulators of histone acetyltransferase p300 [J].
Balasubramanyam, K ;
Swaminathan, V ;
Ranganathan, A ;
Kundu, TK .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (21) :19134-19140
[4]   Covalent modifications of histones during development and disease pathogenesis [J].
Bhaumik, Sukesh R. ;
Smith, Edwin ;
Shilatifard, Ali .
NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2007, 14 (11) :1008-1016
[5]   Epigenetics - An epicenter of gene regulation: Histones and histone-modifying enzymes [J].
Biel, M ;
Wascholowski, V ;
Giannis, A .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (21) :3186-3216
[6]  
Biel M., 2005, ANGEW CHEM, V117, P3248
[7]   Sensing Caspase 3 Activity with Quantum Dot-Fluorescent Protein Assemblies [J].
Boeneman, Kelly ;
Mei, Bing C. ;
Dennis, Allison M. ;
Bao, Gang ;
Deschamps, Jeffrey R. ;
Mattoussi, Hedi ;
Medintz, Igor L. .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (11) :3828-+
[8]   A new method for the detection of ATP using a quantum-dot-tagged aptamer [J].
Chen, Zhang ;
Li, Guang ;
Zhang, Lan ;
Jiang, Junfeng ;
Li, Zhao ;
Peng, Zhihui ;
Deng, Le .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2008, 392 (06) :1185-1188
[9]   Histone acetyl transferases as emerging drug targets [J].
Dekker, Frank J. ;
Haisma, Hidde J. .
DRUG DISCOVERY TODAY, 2009, 14 (19-20) :942-948
[10]   Surface Ligand Effects on Metal-Affinity Coordination to Quantum Dots: Implications for Nanoprobe Self-Assembly [J].
Dennis, Allison M. ;
Sotto, David C. ;
Mei, Bing C. ;
Medintz, Igor L. ;
Mattoussi, Hedi ;
Bao, Gang .
BIOCONJUGATE CHEMISTRY, 2010, 21 (07) :1160-1170
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