Adventitious shoot regeneration from juvenile cotyledons of a biodiesel producing Plant, Jatropha curcas L

被引:30
作者
Khemkladngoen, Naruemon [1 ]
Cartagena, Joyce [2 ]
Shibagaki, Nakako [2 ]
Fukui, Kiichi [1 ]
机构
[1] Osaka Univ, Dept Biotechnol, Grad Sch Engn, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Dept Mat & Life Sci, Grad Sch Engn, Suita, Osaka 5650871, Japan
关键词
Jatropha curcas L; Cotyledon; Plant regeneration; Adventitious shoots; Biodiesel; CULTURES;
D O I
10.1016/j.jbiosc.2010.09.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
An efficient and rapid adventitious shoot regeneration system from young cotyledons of Jatropha curcas L. was developed. After testing several combinations of plant growth regulators, the highest regeneration frequency was obtained for the medium supplemented with 3 mg/l BA and 0.1 mg/l IBA. This system will be useful for promoting genetic improvement efforts in J. curcas. (C) 2010, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码:67 / 70
页数:4
相关论文
共 16 条
[1]  
CARL F, 1993, PLANT CELL TISSUE OR, V33, P105
[2]  
Datta MM, 2007, CURR SCI INDIA, V93, P1438
[3]   High-frequency plant regeneration from leaf-disc cultures of Jatropha curcas L.:: an important biodiesel plant [J].
Deore, Ajay C. ;
Johnson, T. Sudhakar .
PLANT BIOTECHNOLOGY REPORTS, 2008, 2 (01) :7-11
[4]  
Heller J, 1996, PHYS NUT JATROPHA CU
[5]   An evaluation of multipurpose oil seed crop for industrial uses (Jatropha curcas L.):: A review [J].
Kumar, Ashwani ;
Sharma, Satyawati .
INDUSTRIAL CROPS AND PRODUCTS, 2008, 28 (01) :1-10
[6]   Establishment of an Agrobacteriuim-mediated cotyledon disc transformation method for Jatropha curcas [J].
Li, Meiru ;
Li, Hongqing ;
Jiang, Huawu ;
Pan, Xiaoping ;
Wu, Guojiang .
PLANT CELL TISSUE AND ORGAN CULTURE, 2008, 92 (02) :173-181
[7]   In vitro regeneration of Salix nigra from adventitious shoots [J].
Lyyra, S ;
Lima, A ;
Merkle, SA .
TREE PHYSIOLOGY, 2006, 26 (07) :969-975
[8]  
MATAND K, 1997, PLANT SCI, V126, P87
[9]   Evaluation of peanut genotypes for in vitro plant regeneration using thidiazuron [J].
Matand, Kanyand ;
Prakash, C. S. .
JOURNAL OF BIOTECHNOLOGY, 2007, 130 (02) :202-207
[10]   In vitro plant regeneration from immature cotyledon explants of macadamia (Macadamia tetraphylla L. Johnson) [J].
Mulwa, Richard M. S. ;
Bhalla, Prem L. .
PLANT CELL REPORTS, 2006, 25 (12) :1281-1286