Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore

被引：270
作者：
Kojima, H
Hirotani, M
Nakatsubo, N
Kikuchi, K
Urano, Y
Higuchi, T
Hirata, Y
Nagano, T
机构：
[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan
[2] Univ Tokyo, Fac Med, Dept Internal Med 2, Bunkyo Ku, Tokyo 1130033, Japan
来源：
ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 09期
关键词：
Bioimaging;
D O I：
10.1021/ac001136i
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Diaminofluoresceins are widely used for detection and imaging of nitric oxide (NO), but for biological applications, they have the disadvantages that the fluorescence of the fluorescein chromophore is pH-sensitive and overlaps the autofluorescence of cells. We hac-e developed a membrane-permeable fluorescent indicator for NO based on the rhodamine chromophore, DAR-4M AM, which can be excited dth 550-nm light. The fluorescence quantum yield of the product after reaction with NO is 840 times higher than that of DAR-4M. The detection limit of NO nias 7 nM, and the fluorescence showed no pH dependency above pH 4, DAR-4M AM was successfully applied to practical bioimaging of NO produced in bovine aortic endothelial cells.
引用
收藏
页码：1967 / 1973
页数：7
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