Isotope dilution high-performance liquid chromatography -: electrospray tandem mass spectrometry assay for the measurement of 8-oxo-7,8-dihydro-2′-deoxyguanosine in biological samples

被引：149
作者：
Ravanat, JL
Duretz, B
Guiller, A
Douki, T
Cadet, J
机构：
[1] CEA Grenoble, Dept Rech Fondamentale Mat Condensee, SCIB, Lab Lesions Acides Nucl, Grenoble 9, France
[2] Perkin Elmer Appl Biosyst, F-91943 Courtaboeuf, France
来源：
JOURNAL OF CHROMATOGRAPHY B | 1998年 / 715卷 / 02期
关键词：
DNA biomarkers; oxidative stress; 8-oxo-7,8-dihydro-2 '-deoxyguanosine;
D O I：
10.1016/S0378-4347(98)00259-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A sensitive and specific assay aimed at measuring 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) has been developed by associating a reversed-phase liquid chromatographic separation with an electrospray tandem mass spectrometric detection. The HPLC-MS approach in the single ion monitoring (SIM) mode and the HPLC-MS/MS assay in the multiple reaction monitoring (MRM) mode have been compared, using isotopically labeled [M+4] 8-oxodGuo as the internal standard. The limit of detection of 8-oxodGuo was found to be around 5 pmol and 20 fmol for the HPLC-MS and HPLC-MS/MS methods, respectively. The HPLC-MS/MS assay is sensitive enough to allow the determination of the level of 8-oxodGuo in cellular liver DNA and in urine samples. (C) 1998 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：349 / 356
页数：8
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