Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

被引：7
作者：
Sabatini, Maria Elisa [1 ]
Dona, Mattia [1 ,5 ]
Leonetti, Paola [2 ]
Minio, Andrea [3 ]
Delledonne, Massimo [3 ]
Carboneral, Daniela [1 ]
Confalonieri, Massimo [4 ]
Giraffa, Giorgio [4 ]
Balestrazzi, Alma [1 ]
机构：
[1] Dept Biol & Biotechnol L Spallanzani, Via Ferrata 1, I-27100 Pavia, Italy
[2] CNR, Inst Sustainable Plant Protect, Via Amendola 165-A, I-70126 Bari, Italy
[3] Univ Verona, Dept Biotechnol, Str Le Grazie 15, I-37134 Verona, Italy
[4] Fodder & Dairy Prod Res Ctr, Viale Piacenza 29, I-29600 Lodi, Italy
[5] Austrian Acad Sci, GMI, Vienna Bioctr VBC, Dr Bohr Gasse 3, A-1030 Vienna, Austria
来源：
JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2016年 / 58卷 / 07期
关键词：
DNA methylation; Medicago truncatula; RNA-Seq; transposable elements; tyrosyl-DNA phosphodiesterase; TDP1;
D O I：
10.1111/jipb.12457
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The role of plant tyrosyl-DNA phosphodiesterase 1 in genome stability is studied using a Medicago truncatulaMtTdp1-depleted line. Lack of MtTdp1 results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.
引用
收藏
页码：618 / 622
页数：5
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