Depletion of tyrosyl-DNA phosphodiesterase 1α (MtTdp1α) affects transposon expression in Medicago truncatula

被引：7

作者：

Sabatini, Maria Elisa ^{[1
]}

Dona, Mattia ^{[1
,5
]}

Leonetti, Paola ^{[2
]}

Minio, Andrea ^{[3
]}

Delledonne, Massimo ^{[3
]}

Carboneral, Daniela ^{[1
]}

Confalonieri, Massimo ^{[4
]}

Giraffa, Giorgio ^{[4
]}

Balestrazzi, Alma ^{[1
]}

机构：

[1] Dept Biol & Biotechnol L Spallanzani, Via Ferrata 1, I-27100 Pavia, Italy

[2] CNR, Inst Sustainable Plant Protect, Via Amendola 165-A, I-70126 Bari, Italy

[3] Univ Verona, Dept Biotechnol, Str Le Grazie 15, I-37134 Verona, Italy

[4] Fodder & Dairy Prod Res Ctr, Viale Piacenza 29, I-29600 Lodi, Italy

[5] Austrian Acad Sci, GMI, Vienna Bioctr VBC, Dr Bohr Gasse 3, A-1030 Vienna, Austria

来源：

JOURNAL OF INTEGRATIVE PLANT BIOLOGY | 2016年 / 58卷 / 07期

关键词：

DNA methylation; Medicago truncatula; RNA-Seq; transposable elements; tyrosyl-DNA phosphodiesterase; TDP1;

D O I：

10.1111/jipb.12457

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of plant tyrosyl-DNA phosphodiesterase 1 in genome stability is studied using a Medicago truncatulaMtTdp1-depleted line. Lack of MtTdp1 results in a 39% reduction of methylated cytosines as compared to control. RNA-Seq analyses revealed that 11 DNA transposons and 22 retrotransposons were differentially expressed in the Tdp1-2a line. Among them all, DNA transposons (MuDR, hAT, DNA3-11_Mad) and seven retrotransposons (LTR (Long Terminal Repeat)/Gipsy, LTR/Copia, LTR and NonLTR/L1) were down-regulated, while the 15 retrotransposons were up-regulated. Results suggest that the occurrence of stress-responsive cis-elements as well as changes in the methylation pattern at the LTR promoters might be responsible for the enhanced retrotransposon transcription.

引用

页码：618 / 622

页数：5

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