Thiamine biosynthesis in Escherichia coli:: Identification of the intermediate and by-product derived from tyrosine

被引：79

作者：

Kriek, Marco ^{[1
]}

Martins, Filipa ^{[1
]}

Challand, Martin R. ^{[1
]}

Croft, Anna ^{[2
]}

Roach, Peter L. ^{[1
]}

机构：

[1] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

[2] Univ Coll N Wales, Sch Chem, Bangor LL57 2UW, Gwynedd, Wales

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2007年 / 46卷 / 48期

关键词：

biosynthesis; enzyme mechanisms; radicals; thiamine;

D O I：

10.1002/anie.200702554

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) In anaerobic organisms such as E. coli the tyrosine lyase ThiH is essential for the biosynthesis of the thiazole moiety of the vitamin thiamine. ThiH is a member of the radical AdoMet" family. The products formed by cleavage of tyrosine in vitro have been identified and suggest a radical-mediated cleavage resulting in p-cresol and dehydroglycine which is hydrolyzed to glyoxylate. © 2007 Wiley-VCH Verlag GmbH & Co. KGaA."

引用

页码：9223 / 9226

页数：4

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