Robust Encapsulation of Biocompatible Gold Nanosphere Assemblies for Bioimaging via Surface Enhanced Raman Scattering

被引：12

作者：

Schumacher, Marius ^{[1
]}

Jimenez de Aberasturi, Dorleta ^{[2
,3
,4
]}

Merkl, Jan-Philip ^{[1
]}

Scarabelli, Leonardo ^{[2
,3
,5
]}

Lenzi, Elisa ^{[2
,3
]}

Henriksen-Lacey, Malou ^{[2
,3
]}

Liz-Marzan, Luis M. ^{[2
,3
,4
]}

Weller, Horst ^{[1
,6
]}

机构：

[1] Univ Hamburg, Inst Phys Chem, D-20146 Hamburg, Germany

[2] Basque Res & Technol Alliance BRTA, CIC BiomaGUNE, Donostia San Sebastian 20014, Spain

[3] Ctr Invest Biomed Red Bioingn Biomat & Nanomed CI, Donostia San Sebastian 20014, Spain

[4] Basque Fdn Sci, Ikerbasque, Bilbao 48009, Spain

[5] Inst Mat Sci Barcelona ICMAB CSIC, Campus UAB, Bellaterra 08193, Spain

[6] Fraunhofer CAN, D-20146 Hamburg, Germany

来源：

ADVANCED OPTICAL MATERIALS | 2022年 / 10卷 / 14期

基金：

欧洲研究理事会;

关键词：

encapsulation; gold nanoparticles; polymer micelles; Raman reporters; self-assembly; surface-enhanced Raman scattering; COLLOIDAL GOLD; NANOPARTICLES; MONOLAYERS; LIGHT; SIZE; SPECTROSCOPY; COPOLYMERS; STABILITY; NANOSTARS; MOLECULES;

D O I：

10.1002/adom.202102635

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Controlled assembly of gold nanoparticles (AuNPs) into clusters is a promising avenue for the development of sensitive bioimaging and diagnostic tools based on surface-enhanced Raman scattering (SERS). However, several challenges, such as biocompatibility or colloidal and structural stability in biological environments, remain before AuNPs can be used as a tool for in vivo bioimaging. A versatile strategy for the preparation of colloidally stable and biocompatible AuNP clusters (AuNPCs) is introduced with high SERS signals, which are used as SERS contrast bioimaging agents (SERS tags). By tuning the ligand ratio of Raman reporter molecules to stabilizing polymer on the surface of each AuNP, aggregation can be carefully controlled. The resulting AuNPCs exhibit redshifted surface plasmon resonances in the near-infrared (NIR) region, as well as distinct electromagnetic hotspots that give rise to SERS analytical enhancement factors above 10(4), compared to non-clustered spherical AuNPs. Thanks to the protective polymer shell, high levels of cellular uptake with low cytotoxicity are observed, allowing 3D SERS mapping of cells with sufficiently high spatial resolution to detect AuNPCs within intracellular organelles.

引用

页数：10