Direct determination of oxidation state of gold deposits in metal-reducing bacterium Shewanella algae using X-ray absorption near-edge structure spectroscopy (XANES)

被引：26

作者：

Konishi, Yasuhiro

Tsukiyama, Takeshi

Saitoh, Norizoh

Nomura, Toshiyuki

Nagamine, Shinsuke

Takahashi, Yoshio

Uruga, Tomoya

机构：

[1] Osaka Prefecture Univ, Dept Chem Engn, Osaka 5998531, Japan

[2] Hiroshima Univ, Dept Earth & Planetary Syst Sci, Hiroshima 7398526, Japan

[3] Japan Synchrotron Radiat Res Inst, Mikazuki, Hyogo 6795198, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2007年 / 103卷 / 06期

关键词：

metal-reducing bacteria; Shewanella algae; reductive deposition; nanoparticles; gold; X-ray; absorption near-edge structure spectroscopy (XANES);

D O I：

10.1263/jbb.103.568

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

X-ray absorption near-edge structure spectroscopy (XANES) was successfully employed to determine the gold valence in the metal-reducing bacterium Shewanella algae after exposure to a 1 mM aqueous HAuCl(4) solution for 10-120 min. XANES spectra revealed the oxidation state of gold in the bacterial cells to be Au(0) without any contribution from Au(Ill), demonstrating that S. algae cells can reduce AuCl(4)(-) ions to elemental gold. Transmission electron microscopy (TEM) and energy dispersive X-ray (EDX) analysis confirmed that gold nanoparticles 5-15 nm in size were deposited in the periplasmic space of the bacterial cells; a preferable, cell surface location for the easy recovery of biogenic nanoparticles.

引用

页码：568 / 571

页数：4

共 9 条

[1] Intracellular synthesis of gold nanoparticles by a novel alkalotolerant actinomycete, Rhodococcus species [J].