A convenient and sensitive allergy test: IgE crosslinking-induced luciferase expression in cultured mast cells
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Nakamura, R.
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Natl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, JapanNatl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, Japan
Nakamura, R.
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Uchida, Y.
Higuchi, M.
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机构:Natl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, Japan
Higuchi, M.
Nakamura, R.
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Natl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, JapanNatl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, Japan
Nakamura, R.
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Tsuge, I.
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Fujita Hlth Univ, Dept Pediat, Sch Med, Aichi, JapanNatl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, Japan
Tsuge, I.
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Urisu, A.
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Fujita Hlth Univ, Dept Pediat, Sch Med, Aichi, JapanNatl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, Japan
Urisu, A.
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Teshima, R.
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机构:Natl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, Japan
Teshima, R.
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[1] Natl Inst Hlth Sci, Div Novel Foods & Immunochem, Setagaya Ku, Tokyo 1588501, Japan
[2] Fujita Hlth Univ, Dept Pediat, Sch Med, Aichi, Japan
P>Background: For the detection of allergen-specific IgE in sera, solid-phase IgE-binding assays like the CAP test are commonly used. Although such immunochemical methods are very sensitive, they frequently produce false positives. Degranulation of the human IgE receptor (Fc epsilon RI)-transfected rat mast cell (RBL) lines seems to be a possible indicator for human IgE, but spontaneous mediator release from these cells in the presence of human sera is not negligible. Methods: The nuclear factor of activated T-cells (NFAT)-responsive luciferase reporter gene was stably transfected into human Fc epsilon RI-expressing RBL-SX38 cells. One established clone (RS-ATL8) was sensitized with 1 : 100 dilution of sera from patients with egg white allergy and then stimulated with purified or a crude extract of egg white allergen. Results: Sensitization with 15 pg/ml IgE was sufficient to detect IgE crosslinking-induced luciferase expression (EXiLE) by anti-IgE stimulation. Allergen-specific EXiLE was elicited by as little as 1 fg/ml of egg white protein without cytotoxicity. There was a good correlation between results with EXiLE and oral food challenge tests on patients with egg allergy (P = 0.001687, Fisher's exact test). The measured values of EXiLE and the CAP test also correlated well (R = 0.9127, Spearman's test). Conclusion: The EXiLE test using RS-ATL8 cells is a promising in vitro IgE test to evaluate the biological activity of the binding between IgE and allergens.
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Toyokawa City Hosp, Dept Pediat, Toyokawa, JapanFujita Hlth Univ, Teaching Hosp 2, Dept Pediat, Nakagawa Ku, Nagoya, Aichi, Japan
Ando, Hitoshi
Moverare, Robert
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Phadia AB, Uppsala, Sweden
Uppsala Univ, Dept Med Sci Resp Med & Allergol, Uppsala, SwedenFujita Hlth Univ, Teaching Hosp 2, Dept Pediat, Nakagawa Ku, Nagoya, Aichi, Japan
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Bodinier, M.
Brossard, C.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Brossard, C.
Triballeau, S.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Triballeau, S.
Morisset, M.
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Ctr Hosp Univ, Serv Med Interne Immunol Clin & Allergol, Nancy, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Morisset, M.
Guerin-Marchand, C.
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Univ Paris 07, INSERM, U699, Paris, France
Univ Paris 07, Fac Med, Paris, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Guerin-Marchand, C.
Pineau, F.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Pineau, F.
de Coppet, P.
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Univ Nantes, Inst Natl Rech Agron, UMR 1280, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
de Coppet, P.
Moneret-Vautrin, D. A.
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Ctr Hosp Univ, Serv Med Interne Immunol Clin & Allergol, Nancy, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Moneret-Vautrin, D. A.
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Blank, U.
Denery-Papini, S.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
机构:
Toyokawa City Hosp, Dept Pediat, Toyokawa, JapanFujita Hlth Univ, Teaching Hosp 2, Dept Pediat, Nakagawa Ku, Nagoya, Aichi, Japan
Ando, Hitoshi
Moverare, Robert
论文数: 0引用数: 0
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机构:
Phadia AB, Uppsala, Sweden
Uppsala Univ, Dept Med Sci Resp Med & Allergol, Uppsala, SwedenFujita Hlth Univ, Teaching Hosp 2, Dept Pediat, Nakagawa Ku, Nagoya, Aichi, Japan
机构:
Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Bodinier, M.
Brossard, C.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Brossard, C.
Triballeau, S.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Triballeau, S.
Morisset, M.
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Ctr Hosp Univ, Serv Med Interne Immunol Clin & Allergol, Nancy, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Morisset, M.
Guerin-Marchand, C.
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Univ Paris 07, INSERM, U699, Paris, France
Univ Paris 07, Fac Med, Paris, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Guerin-Marchand, C.
Pineau, F.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Pineau, F.
de Coppet, P.
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Univ Nantes, Inst Natl Rech Agron, UMR 1280, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
de Coppet, P.
Moneret-Vautrin, D. A.
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Ctr Hosp Univ, Serv Med Interne Immunol Clin & Allergol, Nancy, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France
Moneret-Vautrin, D. A.
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Blank, U.
Denery-Papini, S.
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Univ Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, FranceUniv Nantes, Inst Natl Rech Agron, Unite Rech 1268, FR-44316 Nantes, France