CRISPR/Cas based gene editing: marking a new era in medical science

被引：13

作者：

Prasad, Kirti ^{[1
,3
]}

George, Anila ^{[1
,2
]}

Ravi, Nithin Sam ^{[1
,2
]}

Mohankumar, Kumarasamypet M. ^{[1
]}

机构：

[1] Ctr Stem Cell Res, Christian Med Coll Campus, Vellore 632002, Tamil Nadu, India

[2] Sree Chitra Tirunal Inst Med Sci & Technol, Thiruvananthapuram 695011, Kerala, India

[3] Manipal Acad Higher Educ, Manipal 576104, Karnataka, India

来源：

MOLECULAR BIOLOGY REPORTS | 2021年 / 48卷 / 05期

关键词：

Programmable nucleases; CRISPR; Cas9; Gene therapy; Gene editing; SEQUENCE-SPECIFIC CONTROL; GENOMIC DNA; GUIDE RNA; CRISPR-CAS9; CAS9; NUCLEASES; ACTIVATION; KNOCKOUT; CLEAVAGE; COMPLEX;

D O I：

10.1007/s11033-021-06479-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CRISPR/Cas9 system, a bacterial adaptive immune system developed into a genome editing technology, has emerged as a powerful tool revolutionising genome engineering in all branches of biological science including agriculture, research and medicine. Rapid evolution of CRISPR/Cas9 system from the generation of double strand breaks to more advanced applications on gene regulation has made the wide-spread use of this technology possible. Medical science has benefited greatly from CRISPR/Cas9; being both a versatile and economical tool, it has brought gene therapy closer to reality. In this review, the development of CRISPR/Cas9 system, variants thereof and its application in different walks of medical science- research, diagnostics and therapy, will be discussed.

引用

页码：4879 / 4895

页数：17

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