Tetrodotoxin Sensitivity of the Vertebrate Cardiac Na+ Current

被引：29

作者：

Vornanen, Matti ^{[1
]}

Hassinen, Minna ^{[1
]}

Haverinen, Jaakko ^{[1
]}

机构：

[1] Univ Eastern Finland, Dept Biol, Joensuu 80101, Finland

来源：

MARINE DRUGS | 2011年 / 9卷 / 11期

基金：

芬兰科学院;

关键词：

evolution of tetrodotoxin sensitivity; vertebrate animals; cardiac sodium current; RESISTANT SODIUM-CHANNELS; ADAPTIVE EVOLUTION; TELEOST FISHES; CHICK HEART; VOLTAGE; BINDING; MUSCLE; DUPLICATIONS; MYOCYTES; RESIDUE;

D O I：

10.3390/md9112409

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Evolutionary origin and physiological significance of the tetrodotoxin (TTX) resistance of the vertebrate cardiac Na+ current (I-Na) is still unresolved. To this end, TTX sensitivity of the cardiac I-Na was examined in cardiac myocytes of a cyclostome (lamprey), three teleost fishes (crucian carp, burbot and rainbow trout), a clawed frog, a snake (viper) and a bird (quail). In lamprey, teleost fishes, frog and bird the cardiac I-Na was highly TTX-sensitive with EC50-values between 1.4 and 6.6 nmol.L-1. In the snake heart, about 80% of the I-Na was TTX-resistant with EC50 value of 0.65 mu mol.L-1, the rest being TTX-sensitive (EC50 = 0.5 nmol.L-1). Although TTX-resistance of the cardiac I-Na appears to be limited to mammals and reptiles, the presence of TTX-resistant isoform of Na+ channel in the lamprey heart suggest an early evolutionary origin of the TTX-resistance, perhaps in the common ancestor of all vertebrates.

引用

页码：2409 / 2422

页数：14

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