Synthesis and characterization of extended length alkyl stationary phases for liquid chromatography with application to the separation of carotenoid isomers

被引：25
作者：
Bell, CM [1 ]
Sander, LC [1 ]
Fetzer, JC [1 ]
Wise, SA [1 ]
机构：
[1] CHEVRON RES & TECHNOL CO,RICHMOND,CA 94802
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 753卷 / 01期
关键词：
stationary phases; LC; carotenoids; carotenes;
D O I：
10.1016/S0021-9673(96)00562-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A new, long-chain (similar to C-34) alkyl-bonded stationary phase for liquid chromatography (LC) was synthesized and utilized for the separation of carotenoid mixtures. This stationary phase exhibited increased selectivity for certain cis isomers of alpha- and beta-carotene as compared to the recently reported C-30 stationary phase. The new stationary phase was synthesized using two bonding strategies: traditional polymeric synthesis and a surface-polymerization synthesis, with the latter yielding columns with improved resolution for carotenoids. A natural extract with a high degree of cis isomerization was well separated with this long-chain stationary phase.
引用
收藏
页码：37 / 45
页数：9
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