Fucoidan Degradation and Preparation of Fuco-oligosaccharides from Saccharina japonica

被引：4
作者：
Geng Lihua [1 ,2 ,3 ]
Jin Weihua [1 ]
Wang Jing [1 ,2 ]
Zhang Quanbin [1 ,3 ]
机构：
[1] Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2017年 / 38卷 / 12期
基金：
中国国家自然科学基金;
关键词：
Fucoidan; Fuco-oligosaccharide; Acid hydrolysis; POLYSACCHARIDES; ANTICOAGULANT;
D O I：
10.7503/cjcu20170232
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The sulfated fuco-oligosaccharides were prepared via acid hydrolysis degradation of crude fucoidan extracted from Saccharina japonica. Through the optimization of degradation and precipitation conditions, the hydrolysis and sedimentation law of fucoidan were studied. Physicochemical analysis showed that the supernatant was heterozygous sulfated fuco-oligosaccharide(fucose>55%). Four fractions of fucose based low polyme-rization degree of oligosaccharides were purified by Bio-Gel P4 low-pressure permeation chromatography, and the fucose content of the fractions was up to 90% according to monosaccharide composition analysis and electrospray ionization mass spectrometry(ESI-MS) analysis. Thus, the fractions were series of low-polymerized sulfated fuco-oligosaccharide/fucose, and the major fractions could be expressed with the formula of Fuc(1 similar to 3)S(1 similar to 3), Fuc(1 similar to 2)S(1 similar to 2), Fuc(1 similar to 2)S, and FucGlcAS.
引用
收藏
页码：2193 / 2197
页数：5
相关论文
共 28 条
  • [1] Structural analysis of a highly sulfated fucan from the brown alga Laminaria cichorioides by tandem MALDI and ESI mass spectrometry
    Anastyuk, Stanislav D.
    Shevchenko, Natalia M.
    Nazarenko, Eugene L.
    Imbs, Tatyana I.
    Gorbach, Vladimir I.
    Dmitrenok, Pavel S.
    Zvyagintseva, Tatyana N.
    [J]. CARBOHYDRATE RESEARCH, 2010, 345 (15) : 2206 - 2212
  • [2] A MODIFIED URONIC ACID CARBAZOLE REACTION
    BITTER, T
    MUIR, HM
    [J]. ANALYTICAL BIOCHEMISTRY, 1962, 4 (04) : 330 - &
  • [3] Outage performance of relay-assisted transmissions in cognitive full-duplex relay networks
    Chen, Hongbin
    Tan, Songgao
    Zhao, Feng
    [J]. EURASIP JOURNAL ON WIRELESS COMMUNICATIONS AND NETWORKING, 2015,
  • [4] A study of fucoidan from the brown seaweed Chorda filum
    Chizhov, AO
    Dell, A
    Morris, HR
    Haslam, SM
    McDowell, RA
    Shashkov, AS
    Nifant'ev, NE
    Khatuntseva, EA
    Usov, AI
    [J]. CARBOHYDRATE RESEARCH, 1999, 320 (1-2) : 108 - 119
  • [5] A NOTE ON DETERMINATION OF ESTER SULPHATE CONTENT OF SULPHATED POLYSACCHARIDES
    DODGSON, KS
    PRICE, RG
    [J]. BIOCHEMICAL JOURNAL, 1962, 84 (01) : 106 - &
  • [6] A COLORIMETRIC METHOD FOR THE DETERMINATION OF SUGARS
    DUBOIS, M
    GILLES, K
    HAMILTON, JK
    REBERS, PA
    SMITH, F
    [J]. NATURE, 1951, 168 (4265) : 167 - 167
  • [7] Neuroprotective Effect of Fucoidan on H2O2-Induced Apoptosis in PC12 Cells Via Activation of PI3K/Akt Pathway
    Gao, Yonglin
    Dong, Chaohua
    Yin, Jungang
    Shen, Jingyu
    Tian, Jingwei
    Li, Chunmei
    [J]. CELLULAR AND MOLECULAR NEUROBIOLOGY, 2012, 32 (04) : 523 - 529
  • [8] THE DETERMINATION OF METHYLPENTOSES
    GIBBONS, MN
    [J]. ANALYST, 1955, 80 (949) : 268 - 276
  • [9] A sulfated polysaccharide, fucans, isolated from brown algae Sargassum vulgare with anticoagulant, antithrombotic, antioxidant and anti-inflammatory effects
    Guerra Dore, Celina Maria P.
    Faustino Alves, Monique Gabriela das C.
    Pofirio Will, Luiza Sheyla E.
    Costa, Thiago G.
    Sabry, Diego A.
    de Souza Rego, Leonardo Augusto R.
    Accardo, Camila M.
    Rocha, Hugo Alexandre O. .
    Filgueira, Luciana Guimaraes A.
    Leite, Edda Lisboa
    [J]. CARBOHYDRATE POLYMERS, 2013, 91 (01) : 467 - 475
  • [10] Relationship between sulfate groups and biological activities of fucans
    Haroun-Bouhedja, F
    Ellouali, M
    Sinquin, C
    Boisson-Vidal, C
    [J]. THROMBOSIS RESEARCH, 2000, 100 (05) : 453 - 459
123