Myricetin affords protection against peroxynitrite-mediated DNA damage and hydroxyl radical formation

被引：49

作者：

Chen, Wei ^{[1
]}

Li, Yudong ^{[1
]}

Li, Jianrong ^{[1
]}

Han, Qiang ^{[1
]}

Ye, Libin ^{[1
]}

Li, Ang ^{[1
]}

机构：

[1] Zhejiang Gongshang Univ, Coll Food Sci & Biotechnol, Hangzhou 310035, Zhejiang, Peoples R China

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2011年 / 49卷 / 09期

基金：

高等学校博士学科点专项科研基金; 中国国家自然科学基金;

关键词：

Myricetin; Peroxynitrite; DNA damage; Hydroxyl radical; CELL-DEATH; REACTIVE OXYGEN; NITRIC-OXIDE; PATHOPHYSIOLOGY; INVOLVEMENT; SUPEROXIDE; GENERATION; INHIBITION; ASTROCYTES; NITRATION;

D O I：

10.1016/j.fct.2011.06.066

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Peroxynitrite has been extensively implicated in the pathogenesis of various forms of neurodegenerative disorders via its cytotoxic effects, this study was undertaken to investigate whether the neuroprotective effect of myricetin is associated with inhibition of peroxynitrite-mediated DNA damage, a critical event leading to peroxynitrite elicited cytotoxicity. We observed that peroxynitrite can cause DNA damage in phi X-174 plasmid DNA and rat primary astrocytes. The presence of myricetin at physiological concentration was found to significantly inhibit DNA strand breakage induced by both peroxynitrite and its generator 3-morpholinosydnonimine (SIN-1). Moreover, the consumption of oxygen caused by SIN-1 was found to be decreased in the presence of myricetin, indicating that myricetin might affect the auto-oxidation of SIN-1. Furthermore, EPR spectroscopy demonstrated that the formation of DMPO-hydroxyl radical adduct (DMPO-OH) from peroxynitrite, and that myricetin inhibited the adduct signal in a concentration-dependent manner. Taken together, these results demonstrate for the first time that myricetin can inhibit peroxynitrite-mediated DNA damage and hydroxyl radical formation. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：2439 / 2444

页数：6

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